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Evaluating the use of Cryopreserved Hepatocytes for the Prediction of In Vivo Hepatic Clearance

Eng, Heather S.

Abstract Details

2004, Master of Science in Pharmaceutical Sciences, University of Toledo, Pharmaceutical Science.
This study examines the clearance of five therapeutic drugs, carbamazepine, phenytoin, procainamide, quinidine, and theophylline, in an in vitro hepatocyte suspension. Parent drug concentration was measured via fluorescence polarization immunoassay or high performance liquid chromatography as a function of time. Parent drug disappearance data were used to calculate linear one-hour disappearance (parent drug activity) and intrinsic in vitro clearance rates. The in vitro clearance value was scaled to a prediction of human in vivo hepatic clearance using the well-stirred model. No correlation between parent drug activity or predicted in vivo hepatic clearance and reported donor characterization was observed. Observed intrinsic in vitro clearance rates were unsuccessful at accurately predicting in vivo hepatic clearance. Therefore, these results are inconclusive regarding the potential use of cryopreserved hepatocytes for the preclinical estimation of human in vivo hepatic clearance.
James Byers (Advisor)

Recommended Citations

Citations

  • Eng, H. S. (2004). Evaluating the use of Cryopreserved Hepatocytes for the Prediction of In Vivo Hepatic Clearance [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1091638312

    APA Style (7th edition)

  • Eng, Heather. Evaluating the use of Cryopreserved Hepatocytes for the Prediction of In Vivo Hepatic Clearance. 2004. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1091638312.

    MLA Style (8th edition)

  • Eng, Heather. "Evaluating the use of Cryopreserved Hepatocytes for the Prediction of In Vivo Hepatic Clearance." Master's thesis, University of Toledo, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1091638312

    Chicago Manual of Style (17th edition)