Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


toledo1147284058.pdf (2.25 MB)

ETD Abstract Container

Abstract Header

Investigation of Skin and Skin Components Using Polarized Fluorescence and Polarized Reflectance Towards the Detection of Cutaneous Melanoma

Yuan, Ye
http://rave.ohiolink.edu/etdc/view?acc_num=toledo1147284058

Abstract Details

2006, Doctor of Philosophy in Engineering, University of Toledo, Bioengineering.
In an effort to investigate viability of using autofluorescence to detect superficial skin cancer (melanoma), polarized fluorescence spectroscopy was employed with the goal of reducing the contribution of the background fluorescence generated from the deep skin. Polarized reflectance was also employed to account for the effects of tissue scattering and absorption on the polarized fluorescence measurements and to measure changes in tissue scattering. An investigation of the skin and its layers using polarized fluorescence spectroscopy has revealed that polarized fluorescence can be generated from the skin, the epidermis, and the dermis using polarized excitation light. The epidermis has the highest retention of fluorescence polarization, while the dermis has the lowest retention of fluorescence polarization. The dependence of the fluorescence anisotropy (a measurement of fluorescence polarization) on dermal thickness was measured, suggesting a role for multiple scattering within the dermis in the depolarization of fluorescence in both the dermis and the skin. A hypothesis of NADH binding change resulting from a metabolic shift in cancer cells was presented. An investigation of normal human melanocytes and melanoma cells using fluorescence anisotropy of the NADH within the cells yielded results consistent with this hypothesis. Normal melanocytes show appreciably higher fluorescence anisotropy than melanoma cells. Since dermal matrix erosion is one of the early stage events in cancer progression, an experimental system was developed to mimic tumor invasion. The process of the enzymatic erosion was investigated with polarized fluorescence, non-polarized fluorescence and polarized reflectance spectroscopy to provide insight into the contrasts between the normal and enzyme-digested dermal matrix. The degradation of the dermal matrix with enzymes results in a decrease in fluorescence emission and light scattering in the matrix. These results confirm the suggestion from literature studies with whole tissue that collagenase-induced dissolution of the extracellular matrix is the cause of the reduction of the fluorescence emission and scattering of the malignant tissue. Fluorescence anisotropy, however, cannot detect the change in the dermal matrix induced by enzyme digestion. It appears that enzymatic digestion does not change the physicochemical properties of the remaining fluorophores and their microenvironment in the dermis.
Patricia Relue (Advisor)
Biophotonics; Fluorescence anisotropy; Autofluorescence; Reflectance; Polarization; Tissue diagnostics; Cell diagnostics; Skin; Cancer detection; Melanoma

Recommended Citations

Citations

  • Yuan, Y. (2006). Investigation of Skin and Skin Components Using Polarized Fluorescence and Polarized Reflectance Towards the Detection of Cutaneous Melanoma [Doctoral dissertation, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1147284058

    APA Style (7th edition)

  • Yuan, Ye. Investigation of Skin and Skin Components Using Polarized Fluorescence and Polarized Reflectance Towards the Detection of Cutaneous Melanoma. 2006. University of Toledo, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1147284058.

    MLA Style (8th edition)

  • Yuan, Ye. "Investigation of Skin and Skin Components Using Polarized Fluorescence and Polarized Reflectance Towards the Detection of Cutaneous Melanoma." Doctoral dissertation, University of Toledo, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1147284058

    Chicago Manual of Style (17th edition)

toledo1147284058
2,148
© 2006, all rights reserved.
This open access ETD is published by University of Toledo and OhioLINK.