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Molecular, morphological, and biogeographic resolution of cryptic taxa in the Greenside Darter Etheostoma blennioidescomplex

Haponski, Amanda E
http://rave.ohiolink.edu/etdc/view?acc_num=toledo1196866050

Abstract Details

2007, Master of Science, University of Toledo, Biology (Ecology).
DNA sequencing has led to the resolution of many cryptic taxa, which are especially prevalent in the North American darter fishes (Family Percidae). The Greenside Darter Etheostoma blennioidescommonly occurs in the lower Great Lakes region, where two putative subspecies, the eastern “Allegheny” type E. b. blennioidesand the western “Prairie” type E. b. pholidotum, overlap. The objective of this study was to test the systematic identity and genetic divergence distinguishing the two subspecies in areas of sympatry and allopatry in comparison to other subspecies and close relatives. DNA sequences from the mtDNA cytochrome bgene and control region and the nuclear S7 intron 1 comprising a total of 1,497 bp were compared from 294 individuals across 18 locations, including the Lake Erie basin and the Allegheny, Meramec, Obey, Ohio, Rockcastle, Susquehanna, and Wabash River systems. Results showed pronounced divergences among taxa ( θ ST= 0.92 – 0.97; p-distance = 0.025 – 0.039) presently designated as E. b. blennioides, E. b. newmanii, and E. b. pholidotum, as well as identification of a fourth clade in the Meramec River. Most traditional morphological characters were significantly different ( P= 0.0001) in distinguishing between E. b. blennioidesand E. b. pholidotum, including scale counts and degree of ventral squamation. However, the range of variation within these characters overlapped, obscuring accurate assignment of individuals to the taxa. The four significantly divergent taxa of the Greenside Darter complex should be evaluated further for potential elevation to species status.
Carol Stepien (Advisor)
80 p.
Biology, Genetics
biogeography; control region; cryptic species; cytochrome b; Etheostoma b. blennioides; Etheostoma b. pholidotum; Greenside Darter; nuclear S7 intron 1

Recommended Citations

Citations

  • Haponski, A. E. (2007). Molecular, morphological, and biogeographic resolution of cryptic taxa in the Greenside Darter Etheostoma blennioidescomplex [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1196866050

    APA Style (7th edition)

  • Haponski, Amanda. Molecular, morphological, and biogeographic resolution of cryptic taxa in the Greenside Darter Etheostoma blennioidescomplex. 2007. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1196866050.

    MLA Style (8th edition)

  • Haponski, Amanda. "Molecular, morphological, and biogeographic resolution of cryptic taxa in the Greenside Darter Etheostoma blennioidescomplex." Master's thesis, University of Toledo, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1196866050

    Chicago Manual of Style (17th edition)

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© 2007, all rights reserved.
This open access ETD is published by University of Toledo and OhioLINK.