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Developmental Toxicity of Ambroxol in Zebrafish Embryos/Larvae: Relevance of SULT-mediated Sulfation of Ambroxol

Al Shaban, Amani
http://rave.ohiolink.edu/etdc/view?acc_num=toledo1271349511

Abstract Details

2010, Master of Science in Pharmaceutical Sciences, University of Toledo, Pharmaceutical Science.
Ambroxol is an active metabolite of bromexine that has been proven to possess a great bronchosecretolytic effect and has been used to treat infants from 0 to 6 month and children till over 12 years of age, as well as adults. My thesis research was aimed to detect potential adverse effects of ambroxol on development using zebrafish embryos/larvae as a model and to investigate the possible involvement of the zebrafish cytosolic sulfotransferases (SULTs) in the protection against the possible adverse effects. Developing eggs at 24 hpf, 48 hpf, and 72 hpf were exposed to different concentrations (1mM, 0.5 mM, 0.25 mM, 0.125 mM, and 0.05 mM) of ambroxol in triplicate and observations were made daily for eleven consecutive days. Ambroxol induced cardiac edema and bradycardia at different stages of development in a dose-dependent manner. Enzymatic assay of purified zebrafish SULTs showed significant sulfation of ambroxol by SULT2 ST1 and SULT3 ST1, 2, 3, 4, and 5. How these SULTs may be involved in protection against the adverse effects of ambroxol remains to be clarified.
Ming-Cheh Liu, PhD (Committee Chair)
Frederick Williams, PhD (Committee Member)
Zahoor Shah, PhD (Committee Member)
57 p.
Pharmacology
ambroxol; cytosolic sulfotranseferases; sulfation; zebrafish developmental toxicity; developmental toxicity

Recommended Citations

Citations

  • Al Shaban, A. (2010). Developmental Toxicity of Ambroxol in Zebrafish Embryos/Larvae: Relevance of SULT-mediated Sulfation of Ambroxol [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1271349511

    APA Style (7th edition)

  • Al Shaban, Amani. Developmental Toxicity of Ambroxol in Zebrafish Embryos/Larvae: Relevance of SULT-mediated Sulfation of Ambroxol. 2010. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1271349511.

    MLA Style (8th edition)

  • Al Shaban, Amani. "Developmental Toxicity of Ambroxol in Zebrafish Embryos/Larvae: Relevance of SULT-mediated Sulfation of Ambroxol." Master's thesis, University of Toledo, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1271349511

    Chicago Manual of Style (17th edition)

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This open access ETD is published by University of Toledo and OhioLINK.