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toledo1302299544.pdf (651.29 KB)
ETD Abstract Container
Abstract Header
Contribution of Biosolids-derived Bioaerosols to the Airborne Microbial Population
Author Info
Lindelof, Kara L.
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=toledo1302299544
Abstract Details
Year and Degree
2011, Master of Science, University of Toledo, Biology (Ecology).
Abstract
Land-application of biosolids is an important means of disposal with possible benefits for soil quality. Bioaerosols might be generated from the application of biosolids and the possible human health risk of pathogenic microorganisms contained in bioaerosols has become an increasingly important issue. Microbial evidence thus far has suggested human health risks are minimal during application of biosolids; however epidemiological studies have reported increased numbers of infection in residents living near biosolids application. Previous studies have focused on the quantification of aerosolized bacteria and the presence of indicator species, while the currenty study uses a DNA-based method to detect alterations to the genetic composition of the aerosolized community following biosolids application. The current study collected soil and aerosol samples from a field site receiving land-applied Class B biosolids. Samples were collected prior to application and over the course of a ten week period following application. Analysis of the data suggested densities of bacteria in aerosol samples did not increase following the application of biosolids. A DNA-based community approach was utilized to investigate the composition of the communities. No change in the community composition was observed after the application of biosolids. Biosolids did not significantly contribute to the airborne population of bacteria in the current study.
Committee
Von Sigler, PhD (Committee Chair)
Farhang Akbar-Khanzadeh, PhD (Committee Member)
Daryl Dwyer, PhD (Committee Member)
Pages
49 p.
Subject Headings
Environmental Science
;
Microbiology
Keywords
biosolids
;
aerosols
;
DGGE
;
denaturing gradient gel electrophoresis
;
bioaerosols
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Citations
Lindelof, K. L. (2011).
Contribution of Biosolids-derived Bioaerosols to the Airborne Microbial Population
[Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1302299544
APA Style (7th edition)
Lindelof, Kara.
Contribution of Biosolids-derived Bioaerosols to the Airborne Microbial Population.
2011. University of Toledo, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1302299544.
MLA Style (8th edition)
Lindelof, Kara. "Contribution of Biosolids-derived Bioaerosols to the Airborne Microbial Population." Master's thesis, University of Toledo, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1302299544
Chicago Manual of Style (17th edition)
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Document number:
toledo1302299544
Download Count:
565
Copyright Info
© 2011, all rights reserved.
This open access ETD is published by University of Toledo and OhioLINK.