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Thesis ~ Eric McDaniel (1).pdf (1.42 MB)

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Organophosphorus Compound Detection Using Enzyme Encapsulated in Peptide Nanotubes

McDaniel, Eric B
http://rave.ohiolink.edu/etdc/view?acc_num=toledo1384526146

Abstract Details

2013, Master of Science in Chemical Engineering, University of Toledo, Chemical Engineering.
Organophosphorus (OP) compounds are commonly used as pesticides as well as nerve agents. These compounds, which are applied in the environment for agricultural and domestic use, must be detected so that residues can be removed to assure safety of humans and the environment. In this research, the goal is to develop a fast, portable, and precise biosensor to detect these OP compounds. To accomplish this, sensors were prepared with enzymes encapsulated in Peptide Nanotubes (PNTs). PNTs were synthesized with diphenylalanine. PNTs can be readily formed by self-assembly of diphenylalanine under mild conditions. Organophosphorus Hydrolase (OPH), an enzyme that has been used to detect OPs, was used in this research as a sensing element to detect malathion, a common OP pesticide. The use of OPH gives a direct method of detecting malathion. Different electrode preparations were used and tested to detect malathion dissolved in water using Cyclic Voltammetry (CV). CV determines current produced by an electro-active species as a response to varying voltage. The increase of a current peak for malathion compared to a blank, on a CV response curve, indicates that there is an electrochemical reaction taking place, presumably due to malathion oxidation facilitated by the OPH enzyme. A linear relationship between the percent increase of peak current versus malathion concentration from 10-100 µM was shown.
Dong-Shik Kim, Dr. (Committee Chair)
Youngwoo Seo, Dr. (Committee Member)
Brent Cameron, Dr. (Committee Member)
Chemical Engineering

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Citations

  • McDaniel, E. B. (2013). Organophosphorus Compound Detection Using Enzyme Encapsulated in Peptide Nanotubes [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1384526146

    APA Style (7th edition)

  • McDaniel, Eric. Organophosphorus Compound Detection Using Enzyme Encapsulated in Peptide Nanotubes. 2013. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1384526146.

    MLA Style (8th edition)

  • McDaniel, Eric. "Organophosphorus Compound Detection Using Enzyme Encapsulated in Peptide Nanotubes." Master's thesis, University of Toledo, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1384526146

    Chicago Manual of Style (17th edition)

toledo1384526146
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© 2013, some rights reserved.Creative Commons License Organophosphorus Compound Detection Using Enzyme Encapsulated in Peptide Nanotubes by Eric B McDaniel is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Toledo and OhioLINK.