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A chemical indirect quantification method for 5-hydroxymethylcytosine

Premnauth, Gurdat
http://rave.ohiolink.edu/etdc/view?acc_num=toledo1470434200

Abstract Details

2016, Master of Science, University of Toledo, Chemistry.
5-Hydroxymethylcytosine (5-hmC) results from the oxidation of 5-methylcytosine (5-mC) by one of the ten-eleven translocation enzymes. This moiety is considered a major epigenetic modification. Although research on DNA methylation is well documented, there is still a lot to learn about DNA hydroxymethylation. Multiples studies have shown the variation of 5-hmC in multiple diseases including a wide range of cancers and neurodegenerative and neurodevelopmental diseases. This fact supports the hypothesis that 5-hmC plays an important role in these diseases, thus making its occurrence a major research target. Although its biological role is still under investigation, multiple quantification methods have been developed to provide a better understanding of its role in different disease states. The method currently under development in our laboratory involves the oxidative labeling of 5-hmC. This is done through the selective oxidation of 5-hmC to 5-formylcytosine (5-foC), using efficient and mild oxidants followed by the formation of a stable conjugate with a biotin aminooxy linker, affinity enrichment, and detection.
Peter Andreana, Dr (Committee Chair)
Amanda Bryant-Friedrich, Dr (Advisor)
113 p.
Chemistry
5-hydroxymethylcytosine; epigenetics; oxidative labeling; DNA modification; quantification

Recommended Citations

Citations

  • Premnauth, G. (2016). A chemical indirect quantification method for 5-hydroxymethylcytosine [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1470434200

    APA Style (7th edition)

  • Premnauth, Gurdat. A chemical indirect quantification method for 5-hydroxymethylcytosine. 2016. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1470434200.

    MLA Style (8th edition)

  • Premnauth, Gurdat. "A chemical indirect quantification method for 5-hydroxymethylcytosine." Master's thesis, University of Toledo, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1470434200

    Chicago Manual of Style (17th edition)

toledo1470434200
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This open access ETD is published by University of Toledo and OhioLINK.