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2017 Masters Thesis Anthony Cekic.pdf (1.36 MB)

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Characterization of Centriolar Protein Poc1

Cekic, Anthony Resul
http://rave.ohiolink.edu/etdc/view?acc_num=toledo1494333292145349

Abstract Details

2017, Master of Science, University of Toledo, Biology (Cell-Molecular Biology).
The centrioles are one of the largest protein-based structures in the cell. A typical centriole consists of nine triplet microtubules as commonly depicted in electron microscopy images in textbooks. Centrioles that don’t adhere to the established typical centriole structure are called atypical centrioles. Sperm centrioles in human and Drosophila have been found to contain both typical and atypical centrioles. Human sperms contain an atypical distal centriole and a typical proximal centriole. Drosophila sperms contain a homologous proximal atypical centrioles called the proximal centriole-like (PCL) and an elongated version of the distal centriole called the giant centriole. Centriolar protein Poc1 has been shown to be essential for the stability and function of centrioles in various animal cells. How Poc1 carries out these functions remains unclear and no adequate tools have been developed to study in this aspect. Here, we show the tools that have been developed to better characterize Poc1 proteins in both Drosophila and human sperms. Firstly, bioinformatics analysis was performed to better understand the Poc1 protein regions in both Drosophila and humans. Secondly, DmPoc1 antibodies were developed and used to observe the localization and protein stability in the sperm centrioles of several DmPoc1 mutants that were discovered through various mutant screens. Using these tools, we found the Poc1 protein regions responsible for DmPoc1 localization and function. Next, several GFP tagged DmPoc1 protein constructs were generated to better understand the effects of DmPoc1 regions on its centriole localization and protein stability. Finally, HsPOC1B antibodies were generated to characterize HsPOC1B localization and protein interactions in human sperms. These data suggest that the tools generated are effective and can be used for future research in Poc1 protein characterization.
Tomer Avidor-Reiss (Committee Chair)
Bruce Bamber (Committee Member)
Guofa Liu (Committee Member)
Song-Tao Liu (Committee Member)
60 p.
Biochemistry; Biology; Molecular Biology
Centriole; POC1; Centriolar Protein; Centriolar protein; Antibody Generation

Recommended Citations

Citations

  • Cekic, A. R. (2017). Characterization of Centriolar Protein Poc1 [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1494333292145349

    APA Style (7th edition)

  • Cekic, Anthony. Characterization of Centriolar Protein Poc1. 2017. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1494333292145349.

    MLA Style (8th edition)

  • Cekic, Anthony. "Characterization of Centriolar Protein Poc1." Master's thesis, University of Toledo, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1494333292145349

    Chicago Manual of Style (17th edition)

toledo1494333292145349
283
© 2017, all rights reserved.
This open access ETD is published by University of Toledo and OhioLINK.