Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


Official Thesis - Final Draft PDF.pdf (484.04 KB)

ETD Abstract Container

Abstract Header

The Impact of FoxO1 Overexpression on the Regulation of CD36 in Skeletal Muscle

Lindsey, Madison L
http://orcid.org/0000-0002-9089-3070
http://rave.ohiolink.edu/etdc/view?acc_num=toledo1525425389232742

Abstract Details

2018, Master of Science, University of Toledo, Exercise Science.
The transcription factor forkhead box O1 (FoxO1) is a downstream effector of insulin and insulin-like growth factor 1 (IGF-1) pathway and regulates various physiological processes including cell proliferation, differentiation, and metabolism. Though the role of FoxO1 in glucose metabolism is well chronicled, its function in lipid metabolism remains to be determined. It has been observed that one of the duties of FoxO1 is to act as a metabolic switch that shifts the metabolic response from utilizing glucose to fatty acids (FA) during times of diminished caloric intake. Downstream targets of FoxO1 aid in the transition from carbohydrate to FA metabolism, and research suggest cluster of differentiation 36 (CD36) to play a key role in the uptake of FA by skeletal muscle.16 The literature indicating a relationship between FoxO1 and the possibly increased expression of CD36 in the plasma membrane is limited and studies have primarily used in vitro models. Therefore, the purpose of this study was to use an in vivo model to elucidate the role of FoxO1 on the regulation of CD36 in skeletal muscle. Transgenic mice overexpressing the muscle-specific FoxO1 protein had their quadriceps muscles excised after an overnight fast. The muscle samples were homogenized, analyzed by western blotting, and quantified using densitometry. The transgenic mice overexpressing the FoxO1 protein had significantly (p<0.05) increased protein expression of FoxO1 compared to the wildtype (WT) mice. However, CD36 expression was not found to be significantly increased in FoxO1 transgenic samples compared with that found in the WT mouse samples. These findings indicate that in vivo increased FoxO1 expression in skeletal muscle is not associated with increased skeletal muscle expression of CD36.
Thomas McLoughlin, Ph.D (Committee Chair)
Francis X. Pizza, Ph.D (Committee Member)
Abraham D. Lee, Ph.D (Committee Member)
30 p.
Physiology
FoxO1, CD36, transcription factor, skeletal muscle, fatty acid metabolism, fatty acids, transgenic mice

Recommended Citations

Citations

  • Lindsey, M. L. (2018). The Impact of FoxO1 Overexpression on the Regulation of CD36 in Skeletal Muscle [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1525425389232742

    APA Style (7th edition)

  • Lindsey, Madison. The Impact of FoxO1 Overexpression on the Regulation of CD36 in Skeletal Muscle. 2018. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1525425389232742.

    MLA Style (8th edition)

  • Lindsey, Madison. "The Impact of FoxO1 Overexpression on the Regulation of CD36 in Skeletal Muscle." Master's thesis, University of Toledo, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1525425389232742

    Chicago Manual of Style (17th edition)

toledo1525425389232742
249
© 2018, all rights reserved.
This open access ETD is published by University of Toledo and OhioLINK.