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Role of Nicotinic Acid Adenine Dinucleotide Phosphate in Memory and Regulatory T Cells

Chakraborty, Anish
http://orcid.org/0000-0003-3732-8563
http://rave.ohiolink.edu/etdc/view?acc_num=toledo1533225900138875

Abstract Details

2018, Doctor of Philosophy, University of Toledo, Medicinal Chemistry.
Nicotinic acid adenine dinucleotide phosphate (NAADP), identified as one of the most potent second messengers, has been studied in different eukaryotic cell types including lymphocytes. Although aspects of NAADP-mediated calcium release in lymphocytes are still under debate, the organelles pertaining to NAADP-mediated calcium release are often characterized as acidic and related to lysosomes. NAADP-mediated calcium release in different subsets of T cells, including naive, effector and natural regulatory T cells, has been studied. However, the effect of NAADP-mediated calcium release has not been widely studied in memory T cells and induced regulatory T cells. The studies being described here had two aims. The first aim consisted of isolation of mouse memory CD4+ T cells from mouse spleen. Using a pharmacological approach, the effect of Ned-19, an NAADP antagonist, on the involvement of NAADP in TCR activation in memory CD4+ T cells and their downstream effector functions, such as proliferation and cytokine production was studied. The second aim consisted of generating induced regulatory T cells in vitro in the presence of NAADP antagonists Ned-19 and BZ194 in order to check their effects on the expression of Foxp3. It also investigated the effect of Ned-19 on proliferation of induced and natural regulatory T cells. Calcium Flux measurement of induced regulatory T cells by flow cytometry was optimized as part of this project.
Katherine Wall (Committee Chair)
James Slama (Committee Member)
Hermann von Grafenstein (Committee Member)
David Giovannucci (Committee Member)
127 p.
Immunology; Pharmacy Sciences

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Citations

  • Chakraborty, A. (2018). Role of Nicotinic Acid Adenine Dinucleotide Phosphate in Memory and Regulatory T Cells [Doctoral dissertation, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1533225900138875

    APA Style (7th edition)

  • Chakraborty, Anish. Role of Nicotinic Acid Adenine Dinucleotide Phosphate in Memory and Regulatory T Cells. 2018. University of Toledo, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1533225900138875.

    MLA Style (8th edition)

  • Chakraborty, Anish. "Role of Nicotinic Acid Adenine Dinucleotide Phosphate in Memory and Regulatory T Cells." Doctoral dissertation, University of Toledo, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1533225900138875

    Chicago Manual of Style (17th edition)

toledo1533225900138875
166
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This open access ETD is published by University of Toledo and OhioLINK.