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DOSE AND VEHICLE EFFECTS ON THE PENETRATION RATE OF SELECTED PLANT POLYPHENOLS THROUGH HUMAN SKIN

BALASUBRAMANIAN, SHREEKRIPA
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1016481382

Abstract Details

2002, MS, University of Cincinnati, Pharmacy : Pharmaceutical Sciences.
Polyphenols, plant phytochemicals have demonstrated a wide variety of beneficial biological activities including astringency, antioxidant, anticarcinogenic and phytoestrogenic properties, to name a few. Due to these attributes, extract of herbs containing these polyphenols have been incorporated in several consumer products. Hence, it is of interest to examine the skin penetration rates of polyphenolic compounds as topical cosmetic agents. The compounds studied kaempferol and catechin, are flavonols and flavanols respectively and are natural antioxidants found in many fruits, vegetables and tea. This study is part of an effort to ascertain the safety and utility of these compounds as skin care ingredients. Doses of 3 H-catechin and 3 H-kaempferol ranging from 0.2-7000 µg/cm 2 were applied to split thickness human cadaver skin mounted on Franz diffusion cells. The vehicle was either 95% ethanol or propylene glycol. Permeation of radiolabel into an aqueous buffer was measured over 72 hrs, and the amount of radioactivity in the receptor fluid was determined by liquid scintillation counting. Results were plotted as amount penetrated vs. time and as percent penetrated vs. time, and were then compared with a theoretical model for skin penetration from a finite dose. Kaempferol penetration was dose-related, in general agreement with the theoretical model. Total penetration increased with increasing dose, ranging from 0.1 µg/cm 2 in 72 h (ethanol, 0.2 µg/cm 2 dose) to 500 µg/cm 2 in 72 h (propylene glycol, 7000 µg/cm 2 dose). Percentage penetration decreased as dose increased, ranging from about 17% / 72 h at the lowest dose to 6.4 % / 72 h at the highest dose. The penetration rate peaked more rapidly than predicted by the model. Catechin penetration from both vehicles was very low (0.5% of dose/ 72 h) and was not dose-related. The reason for this is not yet clear and it may be attributed to chemical modifications of catechin in vehicles over a time period. The human skin penetration rate of kaempferol from solvent-deposited topical applic ations in vitro was in general accord with a physical properties-based model. Penetration of catechin was much lower than anticipated. The results contribute to the understanding of skin permeability of polyphenols such as kaempferol and catechin. We believe they can be extended to other classes of useful botanical ingredients by an appropriate combination of theory and experiments.
Dr. G.B. Kasting (Advisor)
68 p.
Health Sciences, Pharmacy
polyphenols; flavonoids; kaempferol; catechin; skin penetration

Recommended Citations

Citations

  • BALASUBRAMANIAN, S. (2002). DOSE AND VEHICLE EFFECTS ON THE PENETRATION RATE OF SELECTED PLANT POLYPHENOLS THROUGH HUMAN SKIN [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1016481382

    APA Style (7th edition)

  • BALASUBRAMANIAN, SHREEKRIPA. DOSE AND VEHICLE EFFECTS ON THE PENETRATION RATE OF SELECTED PLANT POLYPHENOLS THROUGH HUMAN SKIN. 2002. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1016481382.

    MLA Style (8th edition)

  • BALASUBRAMANIAN, SHREEKRIPA. "DOSE AND VEHICLE EFFECTS ON THE PENETRATION RATE OF SELECTED PLANT POLYPHENOLS THROUGH HUMAN SKIN." Master's thesis, University of Cincinnati, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1016481382

    Chicago Manual of Style (17th edition)

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This open access ETD is published by University of Cincinnati and OhioLINK.