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Chiral Separation Using Capillary Electrophoresis (CE) and Continuous Free Flow Electrophoresis (CFFE)

Liang, Yufu
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1067615432

Abstract Details

2003, MS, University of Cincinnati, Arts and Sciences : Chemistry.
In this report, chiral separations of hydrobenzoin (HB), piperoxan (PI) and methoxyphenamine (MPA) using capillary electrophoresis (CE) are discussed. In the experimental part, separation conditions such as buffer pH, buffer concentration, CE instrumental polarity, capillary length, capillary temperature and sample matrix, etc., were considered and their impact on the chiral separations compared. All selected analytes - hydrobenzoin, piperoxan and methoxyphenamine could be well separated by CE using sulfated β-cyclodextrin (SCD) as chiral additive (CA). Preparative scale separation of MPA and PI was carried out by a continuous free flow electrophoresis (CFFE) instrument using SCD with two different degrees of substitution as CA. Rapid chiral CE methods were developed for the fractionated CFFE samples. Buffer concentration, SCD concentration, applied voltage, sample feeding rate and position, and electrode washing conditions were investigated. Experiments with dyes as surrogates were also completed on the CFFE and UV-Vis instruments. The CFFE instrument PI sample capacity was investigated using optimized conditions.
Dr. Apryll M. Stalcup (Advisor)
101 p.
Chemistry, Analytical
chiral; cyclodextrin; CE; CFFE; separation

Recommended Citations

Citations

  • Liang, Y. (2003). Chiral Separation Using Capillary Electrophoresis (CE) and Continuous Free Flow Electrophoresis (CFFE) [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1067615432

    APA Style (7th edition)

  • Liang, Yufu. Chiral Separation Using Capillary Electrophoresis (CE) and Continuous Free Flow Electrophoresis (CFFE). 2003. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1067615432.

    MLA Style (8th edition)

  • Liang, Yufu. "Chiral Separation Using Capillary Electrophoresis (CE) and Continuous Free Flow Electrophoresis (CFFE)." Master's thesis, University of Cincinnati, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1067615432

    Chicago Manual of Style (17th edition)

ucin1067615432
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© 2003, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.