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ASSESSING THE ENVIRONMENTAL AND BIOLOGICAL IMPLICATIONS OF VARIOUS ELEMENTS THROUGH ELEMENTAL SPECIATION USING INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY

GRANT, TYRE D.
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1085590907

Abstract Details

2004, PhD, University of Cincinnati, Arts and Sciences : Chemistry.
Elements rarely exist as free elements or ions instead they occur as a part of a compound or a species. Toxicological and beneficial effects in the medical and biological field can’t be attributed solely to an element, but to elemental compounds with different biological, physical and chemical properties. Therefore, it is pertinent not to rely on information, which is based solely on total elemental concentration and can be misleading. While there are many separation mechanisms used with inductively coupled plasma mass spectrometry (ICP-MS) for speciation, high performance liquid chromatography (HPLC) was the mode of separation utilized in this work. The coupling of HPLC and ICP-MS is a straightforward technique that allows for the speciation of elements in complex matrices, which may not otherwise be possible. The hybrid of the two techniques provide a robust, sensitive, and selective approach. The bottleneck of speciation analysis often lies at the sample preparation step, the goal being the preservation of species integrity. Improper or incomplete sample preparation can yield misleading results, because of the low tolerance of the ICP-MS for dissolved solids. For this study microwave assisted extraction was one of the techniques used to address this problem. The analyte of choice can be successfully extracted from its matrix with the proper optimization of the microwave parameters, and careful solvent selection. The use of inductively coupled plasma mass spectrometry to study the biological implications of various elements was studied. It was evaluated as a tool for elemental speciation as well as a prescreening tool for the study of larger biomolecules. This was achieved by coupling high performance liquid chromatography (HPLC), and using either the reversed – phase (RP), ion-exchange (IE), or size-exclusion mode (SEC) of separation. More specifically these techniques were used to investigate: the presence of selenium in plants, the distribution of several elements in dietary supplements, the residual occurrence of arsenic compounds in poultry samples, and the incorporation of selenium in selenium-enriched soybeans.
Dr. Joseph Caruso (Advisor)
118 p.
Chemistry, Analytical
Elemental Speciation; ICP-MS; Trace Elemental Analysis

Recommended Citations

Citations

  • GRANT, T. D. (2004). ASSESSING THE ENVIRONMENTAL AND BIOLOGICAL IMPLICATIONS OF VARIOUS ELEMENTS THROUGH ELEMENTAL SPECIATION USING INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1085590907

    APA Style (7th edition)

  • GRANT, TYRE. ASSESSING THE ENVIRONMENTAL AND BIOLOGICAL IMPLICATIONS OF VARIOUS ELEMENTS THROUGH ELEMENTAL SPECIATION USING INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY. 2004. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1085590907.

    MLA Style (8th edition)

  • GRANT, TYRE. "ASSESSING THE ENVIRONMENTAL AND BIOLOGICAL IMPLICATIONS OF VARIOUS ELEMENTS THROUGH ELEMENTAL SPECIATION USING INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY." Doctoral dissertation, University of Cincinnati, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1085590907

    Chicago Manual of Style (17th edition)

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This open access ETD is published by University of Cincinnati and OhioLINK.