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ucin1085756783.pdf (925.31 KB)
ETD Abstract Container
Abstract Header
INFRARED BASED THERMOCYCLING SYSTEM FOR MICROFLUIDIC PCR BIOCHIPS
Author Info
MYNENI, PHALGUN
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1085756783
Abstract Details
Year and Degree
2004, MS, University of Cincinnati, Engineering : Electrical Engineering.
Abstract
A portable thermocycler for polymerase chain reaction (PCR) using an infrared-based heating method has been developed and applied for the DNA amplification in polymer biochips. In the thermocycler, an infrared lamp as a heating source and a thermocouple as a temperature sensor have been adopted. This experiment has evolved a precise temperature control system without the use of an on-chip integrated temperature sensor or heater, which allows us further flexible design of lab-on-a-chips in low cost. A plastic microchip has added advantage of low cost batch fabrication techniques, but its PCR has been considered as one of the difficult tasks due to its low heat transfer coefficient. The microchip is fabricated using Cyclic-Olefin Copolymer (COC) and Polycarbonate (PC) material. To prevent protein adsorption in the channels, Bovine Serum Albumin (BSA) is used in the reaction mixture. This method for DNA amplification shows reasonably good results for PCR in PC chips.
Committee
Dr. Chong Ahn (Advisor)
Pages
50 p.
Keywords
PCR
;
DNA amplification
;
Thermocycler
;
biochips
;
Plastic
;
PC
;
COC
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Citations
MYNENI, P. (2004).
INFRARED BASED THERMOCYCLING SYSTEM FOR MICROFLUIDIC PCR BIOCHIPS
[Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1085756783
APA Style (7th edition)
MYNENI, PHALGUN.
INFRARED BASED THERMOCYCLING SYSTEM FOR MICROFLUIDIC PCR BIOCHIPS.
2004. University of Cincinnati, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1085756783.
MLA Style (8th edition)
MYNENI, PHALGUN. "INFRARED BASED THERMOCYCLING SYSTEM FOR MICROFLUIDIC PCR BIOCHIPS." Master's thesis, University of Cincinnati, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1085756783
Chicago Manual of Style (17th edition)
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Document number:
ucin1085756783
Download Count:
1,612
Copyright Info
© 2004, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.