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Deposition and Patterning of Paraffin Layers for Culture-Based Detection of Mycobacteria in Environmental Samples

Eluru, Hima Bindu
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1093032206

Abstract Details

2004, MS, University of Cincinnati, Engineering : Electrical Engineering.
In this work, paraffin was deposited and patterned for culture-based detection of mycobacteria in environmental samples. To do so, a new method of depositing paraffin using butanol solvent to reduce viscosity of liquid paraffin during spin-coating deposition was explored. Using this approach, 18 mm glass wafers with paraffin layers of 10-70 µm have been successfully fabricated. In an alternative approach, paraffin was mixed with Opticlear solvent and 2-10 µm thick layers of paraffin were deposited on cycloolefin copolymer (COC) plastic wafers. Patterning of paraffin was accomplished in three ways. On glass, paraffin layers were patterned using either wet etching with Opticlear or dry etching using oxygen-based plasma. On COC, paraffin was patterned using lift-off with AZ4620 thick photoresist. The fabricated paraffin patterns were successfully challenged with mycobacteria to ensure that process paraffin layers were still viable. This work is a critical first step in the development of culture-based biochips that use paraffinophilic nature of microorganisms for rapid detection.e
Dr. Ian Papautsky (Advisor)

Recommended Citations

Citations

  • Eluru, H. B. (2004). Deposition and Patterning of Paraffin Layers for Culture-Based Detection of Mycobacteria in Environmental Samples [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1093032206

    APA Style (7th edition)

  • Eluru, Hima Bindu. Deposition and Patterning of Paraffin Layers for Culture-Based Detection of Mycobacteria in Environmental Samples. 2004. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1093032206.

    MLA Style (8th edition)

  • Eluru, Hima Bindu. "Deposition and Patterning of Paraffin Layers for Culture-Based Detection of Mycobacteria in Environmental Samples." Master's thesis, University of Cincinnati, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1093032206

    Chicago Manual of Style (17th edition)

ucin1093032206
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© 2004, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.