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CHARACTERIZATION OF ROOT ENDOPHYTIC BACTERIA

BRANNOCK, JILL MARIE
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1100806990

Abstract Details

2004, MS, University of Cincinnati, Arts and Sciences : Biological Sciences.
Endophyte contribution to contaminant degradation is unknown. Here we determine if bacterial endophytes vary with plant species or contamination, and examine if biodegradation in the soil varies with endophyte types or abundance within the plant. Soils spiked with a mixture of polycyclic aromatic hydrocarbons (PAHs) were planted with either red clover, milkweed, primrose, or left unplanted. Endophytes, extracted and enumerated, were 10X more abundant in clover than in milkweed and primrose in uncontaminated soil. PAH levels had no effect on endophyte abundance. Isolates were identified by sequence analysis. Genera represented in endophyte communities shifted when contamination was present. Rhizosphere PAH degradation (14C mineralization) was positively correlated to initial PAH levels, but planting treatments had no effect. Overall, populations of endophytic bacteria are not random and do respond to external stressors, such as contamination. Endophytic and rhizosphere degradation were unrelated, suggesting these physically separated communities have little impact on one another.
Dr. Jodi Shann (Advisor)
Biology, Microbiology
Endophytes; Mineralization; Contamination; PAH

Recommended Citations

Citations

  • BRANNOCK, J. M. (2004). CHARACTERIZATION OF ROOT ENDOPHYTIC BACTERIA [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1100806990

    APA Style (7th edition)

  • BRANNOCK, JILL. CHARACTERIZATION OF ROOT ENDOPHYTIC BACTERIA. 2004. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1100806990.

    MLA Style (8th edition)

  • BRANNOCK, JILL. "CHARACTERIZATION OF ROOT ENDOPHYTIC BACTERIA." Master's thesis, University of Cincinnati, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1100806990

    Chicago Manual of Style (17th edition)

ucin1100806990
1,464
© 2004, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.