Skip to Main Content
 

Global Search Box

 
 
 
 

ETD Abstract Container

Abstract Header

MOLECULAR AND SEROLOGIC DETECTION OF NEUTROPHIL ANTIGENS AND ANTIBODIES

EMERY, DANIEL L.

Abstract Details

2006, MS, University of Cincinnati, Allied Health Sciences : Transfusion and Transplantation Medicine.
Background: Neutrophil antibodies compromise the innate immune response and can also initiate transfusion related acute lung injury, the number one cause of transfusion related mortality. Neutrophil antibody and antigen detection is technically challenging; few clinical laboratories perform such testing. Our objective was to establish procedures to detect human neutrophil antigens (HNA) and their corresponding antibodies. Methods: Neutrophils were molecularly typed for HNA-1a, and -1b using sequence specific primers (GTI Diagnostics, Waukesha,WI). Results were used to establish two serologic tests, a granulocyte indirect immunofluorescence test (GIFT) and a granulocyte agglutination test (GAT), capable of detecting HNA-1a, 1b, 2a, and 3a. Based on strength of HNA expression in the serologic assays, a neutrophil panel was developed and used to test sera previously screened for anti-HNA by a reference laboratory. Results: GAT reported an Accuracy probability of 97% for HNA-1a and -1b phenotyping and a Positive Predictive value of 100% and 96%, respectively. GIFT reported the HNA-1a and -1b phenotype with an accuracy probability of 97% and 100%, respectively and exhibited 97% and 100% Positive Predictive value. As an antibody screening assay, GIFT demonstrated an average Negative Predictive value of 78% and an average Sensitivity of 81%. Conclusion: This study significantly advanced neutrophil serology at Hoxworth Blood Center. The phenotyping assays demonstrated excellent correlation with the genotypes reported by GranType™ and the antibody screening assay demonstrated good correlation with the results reported by the Blood Center of Wisconsin. Future studies involving antibody identification, through murine monoclonal antibody inhibition or conventional methods should be explored. Also, better elucidation of the antibody screening positive cut-off should be explored in order to maximize the probability of the assay finding no anti-HNA among those who do not have anti-HNA. Increasing the Specificity of anti-HNA screening will enhance the clinical application of the neutrophil antibody screening assay, an important tool in the accurate identification of alloantibody-induced TRALI.
Dr. Brian Susskind (Advisor)
70 p.

Recommended Citations

Citations

  • EMERY, D. L. (2006). MOLECULAR AND SEROLOGIC DETECTION OF NEUTROPHIL ANTIGENS AND ANTIBODIES [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1148243850

    APA Style (7th edition)

  • EMERY, DANIEL. MOLECULAR AND SEROLOGIC DETECTION OF NEUTROPHIL ANTIGENS AND ANTIBODIES. 2006. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1148243850.

    MLA Style (8th edition)

  • EMERY, DANIEL. "MOLECULAR AND SEROLOGIC DETECTION OF NEUTROPHIL ANTIGENS AND ANTIBODIES." Master's thesis, University of Cincinnati, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1148243850

    Chicago Manual of Style (17th edition)