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Detecting Mycobacterium spp. in Hospital Water

Mack, Kristin Lake

Abstract Details

2007, MS, University of Cincinnati, Arts and Sciences : Biological Sciences.
The occurrence of Mycobacterium was determined using traditional culturing methods and a novel device called the Biochip. Mycobacterium spp. were determined to be present in the water collected from every hospital and health care facility that participated (N= 11 sources). In total, fifty-seven species of Mycobacterium were isolated from the hospital water. Of the thirty-four that have been putatively identified by 16S rRNA sequencing, twenty-three (68%) are strains from species that have been implicated as disease-causing agents. The clinically significant species identified include M. avium, M. chelonae, and M. mucogenicum. The Biochip device was able to detect and identify Mycobacterium in seven of eighteen sites sources (39%) tested. The standard methods agreed that there was Mycobacterium present at these seven sources. The device detected no Mycobacterium in five sources where traditional methods also did not. The total agreement of the device to the traditional methods was twelve in eighteen (67%).
Dr. Brian Kinkle (Advisor)
100 p.

Recommended Citations

Citations

  • Mack, K. L. (2007). Detecting Mycobacterium spp. in Hospital Water [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1179331278

    APA Style (7th edition)

  • Mack, Kristin. Detecting Mycobacterium spp. in Hospital Water. 2007. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1179331278.

    MLA Style (8th edition)

  • Mack, Kristin. "Detecting Mycobacterium spp. in Hospital Water." Master's thesis, University of Cincinnati, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1179331278

    Chicago Manual of Style (17th edition)