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HIGH-SENSITIVITY FLUORESCENCE DETECTION FOR LAB-ON-A-CHIP USING CROSS-POLARIZATION AND ORGANIC PHOTODIODES

PAIS, ANDREA
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1187014356

Abstract Details

2007, MS, University of Cincinnati, Engineering : Electrical Engineering.
This work demonstrates and characterizes a novel cross-polarization method of filtering the leakage excitation light from the emission signal of a fluorescent dye. The cross-polarization scheme was incorporated in an integrated on-chip fluorescence detection system, using a metal-halide lamp as excitation source and an organic photodiode as a detector. The benefits of cross-polarization are the significantly-reduced intensity of the leakage excitation light (from 5 µA to 19 nA, or 24 dB), and no wavelength specificity. These benefits make this approach suitable for applications with various dyes, and enable multi-analyte detection. With this setup, detection limits as low as 10 nM of Rhodamine 6G and fluorescein dyes (S/N = 1.5) have been achieved. These results represent a substantial improvement in the detection limit from previous reports in literature using organic photodiodes as fluorescence intensity detectors. This strongly suggests the feasibility of an inexpensive, highly sensitive, compact and disposable lab-on-a-chip fluorescence sensor for point-of-care medical diagnostics, on-site analysis for environmental monitoring and homeland security applications.
Dr. Ian Papautsky (Advisor)
69 p.
Microfluidics; Organic photodiodes; Lab-on-a-chip; on-chip; fluorescence detection; point-of-care diagnostics

Recommended Citations

Citations

  • PAIS, A. (2007). HIGH-SENSITIVITY FLUORESCENCE DETECTION FOR LAB-ON-A-CHIP USING CROSS-POLARIZATION AND ORGANIC PHOTODIODES [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1187014356

    APA Style (7th edition)

  • PAIS, ANDREA. HIGH-SENSITIVITY FLUORESCENCE DETECTION FOR LAB-ON-A-CHIP USING CROSS-POLARIZATION AND ORGANIC PHOTODIODES. 2007. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1187014356.

    MLA Style (8th edition)

  • PAIS, ANDREA. "HIGH-SENSITIVITY FLUORESCENCE DETECTION FOR LAB-ON-A-CHIP USING CROSS-POLARIZATION AND ORGANIC PHOTODIODES." Master's thesis, University of Cincinnati, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1187014356

    Chicago Manual of Style (17th edition)

ucin1187014356
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© 2007, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.