Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


ucin1212062811.pdf (35.3 MB)

ETD Abstract Container

Abstract Header

Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development

SHELTON, ELAINE L.
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1212062811

Abstract Details

2008, PhD, University of Cincinnati, Medicine : Molecular and Developmental Biology.
In the United States, congenital heart defects (CHD) are among the most common birth defects, occurring in approximately 1/100 live births. The majority of CHDs involve abnormal valvuloseptal development. Heart valvulogenesis is initiated by the formation of endocardial cushions, which will ultimately remodel into mature valve leaflets. While much is known about early events in valve development, the molecular mechanisms underlying the transition from endocardial cushion to remodeling valve leaflet remain relatively unknown. Endocardial cushions are composed of highly proliferative, migratory, undifferentiated, mesenchymal valve progenitor cells embedded in a loose extracellular matrix (ECM). The studies herein will demonstrate that robust expression of Twist1, a basic helix-loop-helix transcription factor, and Tbx20, a T-box transcription factor, in response to BMP2 signaling regulates cell proliferation, migration, and mesenchymal gene expression in endocardial cushions. In addition, these studies demonstrate that Twist1 can regulate Tbx20 expression in endocardial cushion cells. Taken together, these findings provide evidence that Twist1 acts upstream of Tbx20 and functions as a high-level regulatory protein in mesenchymal cell proliferation, migration, and differentiation in endocardial cushions. The studies presented in this dissertation identify molecular pathways that govern heart valve development and provide insight into potential therapeutic avenues for those afflicted with congenital valve defects or adult valve disease.
Katherine Yutzey, PhD (Committee Chair)
D. Woodrow Benson, MD, PhD (Committee Member)
Jo El Schultz, PhD (Committee Member)
Timothy Weaver, PhD (Committee Member)
James Wells, PhD (Committee Member)
201 p.
Biomedical Research; Molecular Biology
Twist1; Tbx20; endocardial cushion; development; chicken; heart

Recommended Citations

Citations

  • SHELTON, E. L. (2008). Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1212062811

    APA Style (7th edition)

  • SHELTON, ELAINE. Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development. 2008. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1212062811.

    MLA Style (8th edition)

  • SHELTON, ELAINE. "Thr roles of Twist1 and Tbx20 in endocardial cell proliferation, migration, and differentiation during endocardial cushion development." Doctoral dissertation, University of Cincinnati, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1212062811

    Chicago Manual of Style (17th edition)

ucin1212062811
367
© 2008, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.