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Host-rotaxanes as binding agents: the effects of wheel dynamics

ZHU, JING
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1214400642

Abstract Details

2008, PhD, University of Cincinnati, Arts and Sciences : Chemistry.
Many protein-binding domains have a Hot Spot of 3-5 amino acids and an O-Ring of 20-30 amino acids. The O-ring surrounds the hot spot. To simplify the investigation of protein binding domains, synthetic mimics of proteins are created and investigated. Our host-rotaxanes split a binding domain into two components. A rotaxane contains a linear molecule (axle) threaded through a circular molecule (DB24C8 ring) with bulky molecules (blocking groups) on the ends of the axle to keep the wheel from dethreading. A calixarene, cyclophane or cleft was used as one of the blocking groups to provide a binding pocket, mimicking the hot spot. A ditert-butyl aromatic ring acted as the other blocking group. Functional groups were attached to the DB24C8 ring for guest recognition. The axle contains a positively charged amine group that can interact with the partial negatively charged oxygen atoms on the wheel. This unique arrangement not only provides the opportunity of investigating the relationship between the hot spot and O-ring, but it also simplifies the process of studying the dynamic motion in molecular recognition. To more fully explore the dynamics in molecular recognition, two new cleft-rotaxanes were constructed with very long axles. One axle is a polyether chain, and the other is a polyalkyl chain. The differences in the dynamical motion of these cleft-rotaxanes result in different binding strengths of complexes.
David B. Smithrud, PhD (Committee Chair)
Allan R. Pinhas, PhD (Committee Member)
Apryll Stalcup, PhD (Committee Member)
217 p.
Chemistry
rotaxane

Recommended Citations

Citations

  • ZHU, J. (2008). Host-rotaxanes as binding agents: the effects of wheel dynamics [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1214400642

    APA Style (7th edition)

  • ZHU, JING. Host-rotaxanes as binding agents: the effects of wheel dynamics. 2008. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1214400642.

    MLA Style (8th edition)

  • ZHU, JING. "Host-rotaxanes as binding agents: the effects of wheel dynamics." Doctoral dissertation, University of Cincinnati, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1214400642

    Chicago Manual of Style (17th edition)

ucin1214400642
539
© 2008, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.