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Synthetic Multivalent Glycans for the Detection of Pathogens

Hatch, Duane M.
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1236054639

Abstract Details

2009, PhD, University of Cincinnati, Arts and Sciences : Chemistry.
Current challenges to minimize health risks resulting from a potential bioterrorist attack necessitate the development of autonomous environmental monitoring systems and compact portable biosensors. Most point-of-care diagnostics use antibodies as capture and recognition elements. However, monoclonal antibodies are expensive and exhibit poor shelf life at standard operating temperatures. Unlike antibodies, cell-surface glycans are robust molecules and not prone to facile decomposition. They are natural receptors for several cellular processes such as proliferation, differentiation, migration and changes in cell shape. However, glycans have not been used as recognition motifs in biosensor technologies because of their apparent lack of selectivity. Here, we present that it is possible to tailor glycans to achieve high selectivity and sensitivity. Using a modular synthetic approach, we have synthesized a panel of high-affinity multivalent glycans for the direct detection of type 1 E. coli and the closely related Shiga Toxins (Stx1 and Stx2). Lower limits of detection were achieved with the synthetic glycans compared to commercial antibodies. These robust, synthetic recognition elements can be directly incorporated onto existing biosensor platforms.
Suri Iyer, PhD (Committee Chair)
William Heineman, PhD (Committee Member)
Alison Weiss, PhD (Committee Member)
James Mack, PhD (Committee Member)
226 p.
Organic Chemistry
Glycoconjugates; Glycans; Carbohydrate ligands; Carbohydrate biosensors; Pathogen detection; biotinylated mannose-based ligands

Recommended Citations

Citations

  • Hatch, D. M. (2009). Synthetic Multivalent Glycans for the Detection of Pathogens [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1236054639

    APA Style (7th edition)

  • Hatch, Duane. Synthetic Multivalent Glycans for the Detection of Pathogens. 2009. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1236054639.

    MLA Style (8th edition)

  • Hatch, Duane. "Synthetic Multivalent Glycans for the Detection of Pathogens." Doctoral dissertation, University of Cincinnati, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1236054639

    Chicago Manual of Style (17th edition)

ucin1236054639
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© 2009, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.