Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


ucin1236111208.pdf (2.96 MB)

ETD Abstract Container

Abstract Header

Induction of neurogenesis in the neocortex after ischemic brain injury by manipulation of endogenous neural progenitors

Cancelliere, Alessandro
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1236111208

Abstract Details

2009, PhD, University of Cincinnati, Medicine : Neuroscience/Medical Science Scholars Interdisiplinary.
Many lines of recent studies have demonstrated that endogenous neural stem/progenitor cells (NPCs) contribute to the production of new neurons in selected brain regions following ischemic injury. In other regions, such as the neocortex, regeneration appears to be limited. Previous work in the Nakafuku Lab demonstrated that the migration, survival, and/or differentiation of NPCs from the pPV to the damaged cortical gray matter is restricted. I hypothesized that environmental cues differentially regulate the behavior of NPCs between the adjacent cortex and hippocampus, in which neurogenesis occurs. In order to better characterize the events underlying this restriction of regeneration, I used a rat model of transient forebrain ischemia, in which NPCs resident in the posterior periventricular region (pPV) regenerate hippocampal CA1 pyramidal neurons lost after injury, whereas few new neurons are regenerated in the neocortex. To explore the nature of such environmental cues, I performed microarray analysis for comprehensive gene expression profiling of ischemic brains. These studies identified multiple genes that showed differential expression patterns between the cortex and hippocampus at the time when neurogenesis occurs in the hippocampus. Notably among them were genes encoding insulin-like growth factor (IGF)-I and its regulators, which were highly expressed in the hippocampus, but not the cortex. Administering IGF-I in the cortex of adult ischemic rats, stimulated NPCs in the pPV and increased the number of newborn neurons in the neocortex. These results suggest that IGF-I promotes neurogenesis after ischemia in a region where neurogenesis is otherwise restricted.
Nakafuku Masato, MD, PhD (Committee Chair)
Kenneth Campbell, PhD (Committee Member)
Nancy Ratner, PhD (Committee Member)
Chia Yi Kuan, MD, PhD (Committee Member)
Nadean Brown, PhD (Committee Member)
127 p.
Biomedical Research
neurogenesis; ischemia; neural progenitor cell; neocortex,; IGF-I, stroke

Recommended Citations

Citations

  • Cancelliere, A. (2009). Induction of neurogenesis in the neocortex after ischemic brain injury by manipulation of endogenous neural progenitors [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1236111208

    APA Style (7th edition)

  • Cancelliere, Alessandro. Induction of neurogenesis in the neocortex after ischemic brain injury by manipulation of endogenous neural progenitors. 2009. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1236111208.

    MLA Style (8th edition)

  • Cancelliere, Alessandro. "Induction of neurogenesis in the neocortex after ischemic brain injury by manipulation of endogenous neural progenitors." Doctoral dissertation, University of Cincinnati, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1236111208

    Chicago Manual of Style (17th edition)

ucin1236111208
532
© 2009, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.