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Molecular Microbial Ecology and Operational Evaluation of a Full-scale and Pilot-scale Biologically Active Filter for Drinking Water Treatment

White, Colin P.
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1277154047

Abstract Details

2010, MS, University of Cincinnati, Arts and Sciences : Biological Sciences.
Nitrification in drinking water distribution systems is a problem prevalent throughout the world, and it has become more pertinent since chloramination has become a popular disinfectant technique. Because nitrification requires ammonia, removing ammonia in source waters prior to treatment would benefit both the utility and consumers. Biologically active filtration is a well known technology in Europe but its reliability, and thus implementation, is questioned in the United States. In this study, natural microbial flora from a full-scale treatment plant in Greene County, Ohio was used to seed two pilot scale rapid sand filters. These filters were evaluated for their ability to oxidize ammonia-nitrogen. Molecular techniques, including 16S ribosomal RNA and amoA gene sequencing and denaturing gradient gel electrophoresis (DGGE) analysis, were used to phylogenetically identify and fingerprint the isolates. In addition to investigating nitrification, microbial arsenic oxidation was also investigated in pilot-scale filters. Chemical analysis and microbial ecology is compared and discussed in terms of operational changes and water chemistry.
Ronald Debry, PhD (Committee Chair)
Jodi Shann, PhD (Committee Member)
Darren Lytle, PhD (Committee Member)
112 p.
Microbiology
Biological treatment; Arsenic oxidation; Drinking water; Molecular ecology; Nitrification; Ammonia oxidation

Recommended Citations

Citations

  • White, C. P. (2010). Molecular Microbial Ecology and Operational Evaluation of a Full-scale and Pilot-scale Biologically Active Filter for Drinking Water Treatment [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1277154047

    APA Style (7th edition)

  • White, Colin. Molecular Microbial Ecology and Operational Evaluation of a Full-scale and Pilot-scale Biologically Active Filter for Drinking Water Treatment. 2010. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1277154047.

    MLA Style (8th edition)

  • White, Colin. "Molecular Microbial Ecology and Operational Evaluation of a Full-scale and Pilot-scale Biologically Active Filter for Drinking Water Treatment." Master's thesis, University of Cincinnati, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1277154047

    Chicago Manual of Style (17th edition)

ucin1277154047
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© 2010, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.