Manganese peroxidase, lignin peroxidase, and laccases have shown promise for efficient removal of lignin fraction within the biomass which is resistant to biomass-to-ethanol process. This thesis research study focused on the effectiveness of ligninases, formations of delignification products and optimal experiment conditions for delignification.
Another widely used and studied biomass pretreatment method, dilute acid pretreatment was tested in this study. Four types of biomass were tested. Author focused the studies of kinetic rate, activation energy and pretreated biomass weight model for every model biomass.
Additional, this thesis research conducted a carbon and hydrogen elements analysis for the four model biomass to calculate the mass balance for this dilute acid pretreatment experiment.