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Developing Innovative Metallomics Approaches to Characterize Trace Biometals

Chan, Qilin
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1298044208

Abstract Details

2011, PhD, University of Cincinnati, Arts and Sciences: Chemistry.
Many elements have been claimed as essential for health but their precise roles in life are largely uncharacterized. The increasing interests in the metals/metalloids within biological systems have been driving the fast growth of metallomics, a subject originated to establish the links between metals/metalloids and life. The foundation of metallomics relies on precise determination of biological metal(loid) species. The aim of this dissertation is characterization of such species, primarily metalloproteins, in various biological systems using innovative metallomics approaches. These include several multi-technique methods in which the traditional elemental speciation with HPLC-ICPMS (inductively coupled plasma mass spectrometry) and LC-MS/MS based proteomics were combined to meet the challenges of metalloprotein characterization in animals and plants. Two biologically important elements were intensively studied, zinc (Zn) in murine macrophages and selenium (Se) in plants. The metallomics study in macrophages revealed that Zn played a fundamental role in host defense against Histoplasma capsulatum infection, and a few Zn-binding proteins selectively responded to macrophage activation or the pathogen infection. The Se study elucidated the complete profiles of Se metabolites in kale (Brassica oleracea) and soybean (Glycine max) and more importantly, it discovered the first Se-containing protein in plants, the Se-containing Bowman-Birk proteinase isoinhibitor D-II [Glycine max] derived from the Se-enriched soybean. These findings not only opened new avenues to study Zn’s precise roles in immunity and Se-containing proteins in plants, but also lead the interactions between metallomics and other research areas, such as immunology and proteomics.
Joseph Caruso (Committee Chair)
Anna Gudmundsdottir (Committee Member)
Patrick Limbach (Committee Member)
139 p.
Analytical Chemistry
Metallomics; ICPMS; Macrophage; Soybean; Kale; Speciation

Recommended Citations

Citations

  • Chan, Q. (2011). Developing Innovative Metallomics Approaches to Characterize Trace Biometals [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1298044208

    APA Style (7th edition)

  • Chan, Qilin. Developing Innovative Metallomics Approaches to Characterize Trace Biometals. 2011. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1298044208.

    MLA Style (8th edition)

  • Chan, Qilin. "Developing Innovative Metallomics Approaches to Characterize Trace Biometals." Doctoral dissertation, University of Cincinnati, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1298044208

    Chicago Manual of Style (17th edition)

ucin1298044208
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© 2011, some rights reserved.Creative Commons License Developing Innovative Metallomics Approaches to Characterize Trace Biometals by Qilin Chan is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Cincinnati and OhioLINK.