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Nanofiber-based therapy for diabetic wound healing: a mechanistic study

Cho, Hongkwan
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1352402217

Abstract Details

2012, PhD, University of Cincinnati, Engineering and Applied Science: Biomedical Engineering.
Chronic diabetic wounds are a serious complication of diabetes mellitus, representing more than 27% of total annual diabetic health care costs which exceed $116 billion annually in the US alone. Diminished infiltration of new blood vessels (neovascularization) into the wound is a major factor contributing to the impaired healing of diabetic ulcers. A previous study has shown that the wounds treated with RAD16-II peptide nanofibers resulted in increased infiltration of both endothelial cells (ECs) and endothelial progenitor cells (EPCs) into the wounds in diabetic mouse model, resulting in enhanced neovascularization and accelerated wound healing. However, in order to utilize the full potential of the peptide nanofibers for treatment of diabetic ulcers, it is necessary to understand the mechanisms by which it works. The long-term goal of this research is to develop a therapy to create a wound microenvironment which enhances neovascularization and overall healing in diabetic wounds by utilizing peptide nanofibers. The studies in this dissertation contribute to this goal by investigating the nature of interactions between ECs and nanofibers, as well as the role of bone marrow-derived EPCs during nanofiber-mediated neovascularization. The central hypothesis of this research is that the RAD16-II peptide nanofibers enhance wound neovascularization and improve diabetic wound healing by regulating both angiogenesis (by ECs) and vasculogenesis (by EPCs). The results from this dissertation research help identify the roles of ECs and EPCs during nanofiber-mediated neovascularization. The results from this study demonstrate that the nanofibers promote wound neovascularization mostly by increasing EC recruitment via an integrin dependent mechanism, with a limited contribution from bone marrow-derived EPCs. The findings of this study will contribute towards developing an optimal microenvironment to enhance diabetic wound neovascularization and for vascular tissue engineering applications.
Daria Narmoneva, PhD (Committee Chair)
Christy Holland, PhD (Committee Member)
Jason Shearn, PhD (Committee Member)
164 p.
Biomedical Research
Angiogenesis; vasculogenesis; diabetic wound; self-assembling peptide nanofibers; endothelial progenitor cells; ;

Recommended Citations

Citations

  • Cho, H. (2012). Nanofiber-based therapy for diabetic wound healing: a mechanistic study [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1352402217

    APA Style (7th edition)

  • Cho, Hongkwan. Nanofiber-based therapy for diabetic wound healing: a mechanistic study. 2012. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1352402217.

    MLA Style (8th edition)

  • Cho, Hongkwan. "Nanofiber-based therapy for diabetic wound healing: a mechanistic study." Doctoral dissertation, University of Cincinnati, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1352402217

    Chicago Manual of Style (17th edition)

ucin1352402217
604
© 2012, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.