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Influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model

Goenka, Shilpi
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1378195712

Abstract Details

2013, MS, University of Cincinnati, Engineering and Applied Science: Materials Science.
Hand-Arm Vibration Syndrome (HAVS) is caused by hand-transmitted vibration in industrial workers and consists of vascular and musculoskeletal disorders. Current International Standard Organization (ISO) guidelines might underestimate vascular injury associated with range of vibration frequencies near resonance. Also, delineation of the response of bone tissue under different frequencies of vibration will allow formulation of more accurate guidelines for musculoskeletal disorders (MSD) linked to HAVS and eventually for its therapeutic outcome. A rat-tail model is used to investigate the effects of higher frequencies > 100 Hz on early vascular damage and bone changes. Two separate set of experiments were conducted to assess changes in artery tissue and bone under vibration. For experiment 1, 13 male Sprague Dawley rats (250 ± 15 gm) were used. Rat-tails were vibrated at 125 Hz and 250 Hz (49 m/s2) for 1D, 5D and 10D; D=days (4hr/day). Structural damage of vessels was quantified by vacuole count using Toluidine blue staining whereas biochemical changes were assessed by nitrotyrosine (NT) staining. The results were analyzed using one-way repeated measures mixed model ANOVA at p< 0.05 level of significance. For experiment 2, 24 Male Sprague Dawley rats (250 ± 15 gm) were used to investigate the effects of higher frequencies >100 Hz and duration of vibration (1D, 5D and 20D; D=days) on bone tissue. Rat-tails were vibrated at 125 Hz and 250 Hz (49 m/s2) for 1D, 5D and 20D (4hr/day). Structural damage of bone was quantified using histological staining. The biochemical changes were assessed by mineralization changes (material tests) and nitrotyrosine (NT) staining. The results were analyzed using one-way repeated measures mixed model ANOVA at p< 0.05 level of significance. For the artery tissue, the structural damage peaked at 125 Hz causing significant vacuoles (40.62 ± 9.8) (compared to control group (8.36 ± 2.49)) and reduced at 250 Hz (12.33 ± 2.98). However, the biochemical damage (NT signal) increased significantly for 125 Hz (143.35 ± 5.8) and for 250 Hz (155.8 ± 7.35) compared to the control group (101.7 ± 4.18). Our results demonstrate that vascular damage in the form of structural and biochemical disruption is significant at 125 Hz and 250 Hz. Hence the current ISO guidelines might underestimate vascular damage at frequencies > 100 Hz. For the bone, structural damage in cortical bone was significant at 250 Hz while the structural damage in the trabecular bone showed a moderate significance at 125 Hz and 250 Hz. The biochemical damage was significant at both the 125 Hz and 250 Hz vibration frequencies. Also, the structural damage was significant at 1D and 20D for the trabecular bone, while it was significant at 5D for cortical bone. Also, the biochemical damage was significant at all the time points of vibration (1D, 5D and 20D). Our results demonstrate that bone alterations in the form of structural and biochemical disruption in bone tissue are significant at 125 Hz and 250 Hz. The duration of vibration also has a significant effect.
Rupak Banerjee, Ph.D., P.E. (Committee Chair)
Keith F. Stringer, M.Sc. (Committee Member)
Relva Buchanan, Sc.D. (Committee Member)
J. Kim, Ph.D. (Committee Member)
Rodney Roseman, Ph.D. (Committee Member)
87 p.
Engineering
nitrotyrosine; hand arm vibration; rat-tail model; vascular damage; osteocyte; bone

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Citations

  • Goenka, S. (2013). Influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1378195712

    APA Style (7th edition)

  • Goenka, Shilpi. Influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model. 2013. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1378195712.

    MLA Style (8th edition)

  • Goenka, Shilpi. "Influence of higher frequency components and duration of mechanical vibration on artery and bone in a rat-tail model." Master's thesis, University of Cincinnati, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1378195712

    Chicago Manual of Style (17th edition)

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This open access ETD is published by University of Cincinnati and OhioLINK.