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Seasonal Dynamics and Relative Persistence Potential of the Enteric Species of Enterovirus in Wastewater

Brinkman, Nichole E
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1396531472

Abstract Details

2014, PhD, University of Cincinnati, Arts and Sciences: Biological Sciences.
Human enteroviruses (EV) are a large group of enteric pathogens containing approximately 104 serotypes, which cluster into four different species (EV-A-D). They can be transmitted from infected to susceptible individuals via a person-to-person route, contaminated food and water used for recreation, shellfish harvesting, or drinking. Assessments of public health risk due to exposure to waterborne enteroviruses require, in part, an understanding of the levels of these pathogens in water sources. Two key factors in determining their occurrence in water are 1) knowing the extent of the diversity of this group present in a particular water source and 2) the ability of individual members to persist in water. Data published to date regarding presence and persistence potential of enteroviruses has been limited to poliovirus, and members of one of the four enterovirus species, leaving the majority of serotypes uncharacterized. The objectives for this project were to assess the diversity of enteroviruses present in wastewater and evaluate the influence that the persistence potential may have on the diversity profile. Towards this end, a method to concentrate representative enterovirus species was identified and determined to equally recover enterovirus species from primary effluent of wastewater. This method was then applied to monthly wastewater samples collected locally over a one year period for deep sequencing of enteroviruses present. This culture-independent, next-generation sequencing approach allowed for hundreds to thousands of enterovirus genomic sequences in each sample to be identified. Analysis of these sequences revealed that members of EV-A, EV-B and EV-C are present in each month of the year, while EV-D is present sporadically. EV-C is present in relatively low abundance year-round, while EV-A and EV-B alternate in predominance in a pattern that coincides with season. Investigation into the persistence potential of enteroviruses in wastewater shows that persistence cannot be described by species, but is serotype dependent. Poliovirus (EV-C) and enterovirus 70 (EV-D) were observed to be most persistent, while CVA10 (EV-A) was least persistent. The members of EV-B examined (CVA9, CVB1 and echovirus 30) showed moderate levels of persistence. The work described here provides a new perspective of the diversity profile of enterovirus present in wastewater and begins to decode the relative persistence potential of, until now, unrepresented members.
Brian Kinkle, Ph.D. (Committee Chair)
G. Shay Fout, Ph.D. (Committee Member)
Eric Villegas, Ph.D. (Committee Member)
Nicholas Ashbolt, Ph.D. (Committee Member)
Dennis Grogan, Ph.D. (Committee Member)
165 p.
Virology
enterovirus; wastewater; persistence

Recommended Citations

Citations

  • Brinkman, N. E. (2014). Seasonal Dynamics and Relative Persistence Potential of the Enteric Species of Enterovirus in Wastewater [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1396531472

    APA Style (7th edition)

  • Brinkman, Nichole. Seasonal Dynamics and Relative Persistence Potential of the Enteric Species of Enterovirus in Wastewater. 2014. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1396531472.

    MLA Style (8th edition)

  • Brinkman, Nichole. "Seasonal Dynamics and Relative Persistence Potential of the Enteric Species of Enterovirus in Wastewater." Doctoral dissertation, University of Cincinnati, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1396531472

    Chicago Manual of Style (17th edition)

ucin1396531472
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This open access ETD is published by University of Cincinnati and OhioLINK.