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Retention, Regrowth, and Washout of Escherichia coli in Mixed Species Biofilms Formed from Dechlorinated Cincinnati Tap Water in a Laboratory Annular Reactor System

Mathure, Mugdha
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1416570778

Abstract Details

2014, MS, University of Cincinnati, Engineering and Applied Science: Environmental Engineering.
Pathogens in drinking water have long been known to cause diseases in humans making it necessary to ensure drinking water quality. Microorganisms attach to the surface of pipes and form biofilms in drinking water distribution systems. Diffusion of disinfectants inside the biofilm decreases as the thickness of biofilm increases. In such cases, the biofilms can act as reservoirs for pathogens and help them colonize. Therefore, the number of suspended coliforms detected during routine monitoring is not an accurate reflection of the concentration of contaminants in a water distribution system. Moreover, the pathogens may be reintroduced in the distribution system when the biofilm detaches from the pipe surface. In this study, biofilms were allowed to develop on polycarbonate coupons inside laboratory biofilm annular reactors. After the development of biofilms on the coupons, the reactor was spiked with fluorescently labeled E. coli. The retention, penetration ability, and washout of E. coli from the biofilms were analyzed in this study. E. coli penetrated to the surface of the substratum and persisted in the biofilms for ~ 10 days.
Margaret Kupferle, Ph.D. P.E. (Committee Chair)
Benjamin Vaughan, Ph.D. (Committee Member)
David Wendell, Ph.D. (Committee Member)
65 p.
Environmental Engineering
biofilm; E coli; confocal microscopy; drinking water

Recommended Citations

Citations

  • Mathure, M. (2014). Retention, Regrowth, and Washout of Escherichia coli in Mixed Species Biofilms Formed from Dechlorinated Cincinnati Tap Water in a Laboratory Annular Reactor System [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1416570778

    APA Style (7th edition)

  • Mathure, Mugdha. Retention, Regrowth, and Washout of Escherichia coli in Mixed Species Biofilms Formed from Dechlorinated Cincinnati Tap Water in a Laboratory Annular Reactor System. 2014. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1416570778.

    MLA Style (8th edition)

  • Mathure, Mugdha. "Retention, Regrowth, and Washout of Escherichia coli in Mixed Species Biofilms Formed from Dechlorinated Cincinnati Tap Water in a Laboratory Annular Reactor System." Master's thesis, University of Cincinnati, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1416570778

    Chicago Manual of Style (17th edition)

ucin1416570778
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© 2014, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.