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Understanding the Cellular Mechanisms of the Leukocyte Adhesion Deficiency Type III Disorder with the Use of Patient Induced Pluripotent Stem Cells

Chai, Yi Wen
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1417784194

Abstract Details

2014, MS, University of Cincinnati, Allied Health Sciences: Transfusion and Transplantation Sciences.
Leukocyte Adhesion Defiency type III (LAD-III) is a rare congenital defect that affects the normal adhesive function of phagocytic cells of the myeloid lineage. Bone marrow from a patient with LAD-III was obtained and used to generate induced pluripotent stem cells (iPSCs). The iPSCs were determined to have normal karyotype, phenotype, differentiation capacity, cell surface markers, self-renewal capabilities and pluripotency. We hypothesize that iPSCs from this patient can be used to generate hematopoietic cells. In addition, the generated hematopoietic cells will have the same characteristics as the patient cells. We also evaluated the hematopoietic cells generated from the iPSCs, including their number and differentiation status, and their ability to migrate and adhere in response to inflammatory mediators. The main purpose of this project is to successfully model the cellular functional defect in LAD-III using iPS derived hematopoietic cells for in vitro cell culture analysis. Our results showed that we were able to generate hematopoietic progenitor cells and myeloid cells from our patient derived iPSCs. Furthermore, the differentiated myeloid cells had sustained the expected LAD-III functional defects of cellular adhesion and migration. In summary, this study was able to show the functional cellular defects in LAD-III by using patient-specific iPSCs to model the disease in vitro.
Carolyn Lutzko, Ph.D. (Committee Chair)
Jose Cancelas-Perez, M.D. (Committee Member)
Thomas Leemhuis, Ph.D. (Committee Member)
61 p.
Molecular Biology
LAD-III; induced pluripotent stem cells; iPSC; hematopoietic cells; congenital; Leukocyte Adhesion Defiency

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Citations

  • Chai, Y. W. (2014). Understanding the Cellular Mechanisms of the Leukocyte Adhesion Deficiency Type III Disorder with the Use of Patient Induced Pluripotent Stem Cells [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1417784194

    APA Style (7th edition)

  • Chai, Yi Wen. Understanding the Cellular Mechanisms of the Leukocyte Adhesion Deficiency Type III Disorder with the Use of Patient Induced Pluripotent Stem Cells. 2014. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1417784194.

    MLA Style (8th edition)

  • Chai, Yi Wen. "Understanding the Cellular Mechanisms of the Leukocyte Adhesion Deficiency Type III Disorder with the Use of Patient Induced Pluripotent Stem Cells." Master's thesis, University of Cincinnati, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1417784194

    Chicago Manual of Style (17th edition)

ucin1417784194
446
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This open access ETD is published by University of Cincinnati and OhioLINK.