Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


12759.pdf (1.8 MB)

ETD Abstract Container

Abstract Header

Targeting IL-4 locus for epigenetic reprogramming

Oksuz, Samet
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1423581203

Abstract Details

2014, MS, University of Cincinnati, Medicine: Immunology.
Naive T cells differentiate into several different helper populations in response to cytokine milieu and pathogens. Regulation of this differentiation is important for mounting an effective immune response and preventing autoimmune diseases; for instance, abnormal Treg differentiation leads to tolerance against tumors and Th1 and Th2 differentiation may lead to autoimmune diseases and asthma, respectively. There are several studies showing that there is a correlation between epigenetic marks on signature genes and differentiation into distinct T cell lineages. Results from Wei et al. (2009) and Hawkins et al. (2013) show that positive H3K4me marks are enriched at the IL-4 locus in Th2 cells. Our preliminary data also show that the positive marks appear at lineage-specific cytokine genes in memory cells. Our aim is to determine whether this correlation reflects a causative relationship between presence of positive histone marks and T cells’ ability to express cytokines. In this study, we are planning to deposit histone modifications at the IL-4 gene in a locus specific manner. In order to do that, we are developing TALEMs, fusion proteins of TALE-based DBD and a catalytic domain of a histone modifying enzyme. Here we are describing development of TALE DBDs to target human and mouse IL4 genes and a set of experiments aiming at validating these TALE fusion proteins.
Jonathan Katz, Ph.D. (Committee Chair)
Artem Barski, Ph.D. (Committee Member)
David Hildeman, Ph.D. (Committee Member)
43 p.
Immunology
epigenetics; TALE; helper T cell; Il4

Recommended Citations

Citations

  • Oksuz, S. (2014). Targeting IL-4 locus for epigenetic reprogramming [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1423581203

    APA Style (7th edition)

  • Oksuz, Samet. Targeting IL-4 locus for epigenetic reprogramming. 2014. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1423581203.

    MLA Style (8th edition)

  • Oksuz, Samet. "Targeting IL-4 locus for epigenetic reprogramming." Master's thesis, University of Cincinnati, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1423581203

    Chicago Manual of Style (17th edition)

ucin1423581203
905
© 2014, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.