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The Role of Urinary Cell-free MicroRNA's as Biomarkers of Lupus Nephritis in Children

Abulaban, Khalid M
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1427962725

Abstract Details

2015, MS, University of Cincinnati, Medicine: Clinical and Translational Research.
Background: There is a dire need of non-invasive biomarkers of lupus nephritis (LN) activity. MicroRNAs (miRNAs) are endogenous, non-coding, single-stranded RNAs involved in the regulation of host genome expression at the post-transcriptional level that play a role in autoimmune disease. The objective of this study is to investigate the relationship of select urinary cell-free microRNA's in children with active LN vs. extra-renal systemic lupus erythematosus (SLE) vs. controls to the LN-Panel [neutrophil gelatinase associated lipocalin (NGAL), monocyte chemotactic protein 1(MCP1), transferrin (Tf), Beta-trace protein (BTP)] and traditional LN biomarkers. Methods: Select miRNA’s were measured using real-time polymerase chain reaction in the urine pellet (UP) and supernatant (SUP) in 14 patients with active LN ,10 with extra-renal SLE and 10 controls (five juvenile idiopathic arthritis and five fibromyalgia patients) prospectively every 3 months. Disease activity was measured by the systemic lupus erythematosus disease activity index (SLEDAI). Urine samples were assayed for the LN-Panel and traditional biomarkers were recorded. Results: MiR-125a, miR-150, and miR-155 in the SUP had moderate to good positive correlation with all LN-Panel (r = 0.35 – 0.68), but had weak correlation with SLEDAI and the traditional biomarkers (r-<0.3). On the other hand measurement of the miRNA’s in the UP did not correlate with any measure. Conclusion: The urinary supernatant of miRNAs miR-125a, miR-150, and miR-155 are differentially excreted in the urine with active LN. Our evaluation suggests that cell-free urinary miRNA complement, but not superior to the newer LN Panel in their ability to assess concurrent LN activity.
Erin Nicole Haynes, Dr.P.H. (Committee Chair)
Hermine Brunner, M.D. (Committee Member)
Michael Bennett, Ph.D. (Committee Member)
Jun Ying, Ph.D. (Committee Member)
21 p.
Surgery
SLE; lupus nephritis; biomarker; microRNA

Recommended Citations

Citations

  • Abulaban, K. M. (2015). The Role of Urinary Cell-free MicroRNA's as Biomarkers of Lupus Nephritis in Children [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1427962725

    APA Style (7th edition)

  • Abulaban, Khalid. The Role of Urinary Cell-free MicroRNA's as Biomarkers of Lupus Nephritis in Children. 2015. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1427962725.

    MLA Style (8th edition)

  • Abulaban, Khalid. "The Role of Urinary Cell-free MicroRNA's as Biomarkers of Lupus Nephritis in Children." Master's thesis, University of Cincinnati, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1427962725

    Chicago Manual of Style (17th edition)

ucin1427962725
539
© 2015, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.