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Global Identification and Mass Mapping of tRNA Isoacceptors Using Targeted Tandem Mass Spectrometry

Abstract Details

2015, PhD, University of Cincinnati, Arts and Sciences: Chemistry.
This dissertation is focused on developing and implementing liquid chromatography-tandem mass spectrometry (LC-MS/MS) approaches for the high-throughput characterization of small RNAs in complex biological matrices. Non-coding ribonucleic acids (ncRNA) have become increasingly recognized as important to a number of biological functions, such as gene expression at both the transcriptional and translational levels. Among the many classes of ncRNAs, transfer ribonucleic acids (tRNA) are noted for their significant role in the center of the central dogma of molecular biology, translating the genetic code into functional proteins. Naturally occurring chemical modifications are applied post-transcriptionally to these polymers giving them added structure and function. Despite biological relevance, the complex nature of mixtures containing a pool of total tRNA typically requires lengthy, impractical sample preparations steps. The “digest and go” methods proposed in this dissertation should be capable of helping advance the field of RNomics, much like the lasting impact LC-MS based strategies have had on the rapidly advancing field of proteomics; lending themselves to applications from basic molecular biology studies to clinical applications previously thought impractical. These advances were accomplished by implementing targeted MS/MS based assays, which take advantage of high-through-put data collection, taking one third the time of previous LC-MS based approaches and capable of identifying more individual tRNA isoacceptors, in conjunction with automated data analysis.
Patrick Limbach, Ph.D. (Committee Chair)
Edward Merino, Ph.D. (Committee Member)
Thomas Ridgway, Ph.D. (Committee Member)
Apryll Stalcup, Ph.D. (Committee Member)
100 p.

Recommended Citations

Citations

  • Wetzel, C. (2015). Global Identification and Mass Mapping of tRNA Isoacceptors Using Targeted Tandem Mass Spectrometry [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1448037316

    APA Style (7th edition)

  • Wetzel, Collin. Global Identification and Mass Mapping of tRNA Isoacceptors Using Targeted Tandem Mass Spectrometry. 2015. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1448037316.

    MLA Style (8th edition)

  • Wetzel, Collin. "Global Identification and Mass Mapping of tRNA Isoacceptors Using Targeted Tandem Mass Spectrometry." Doctoral dissertation, University of Cincinnati, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1448037316

    Chicago Manual of Style (17th edition)