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Inertial microfluidic vortex cell sorter

Wang, Xiao
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1458643998

Abstract Details

2016, PhD, University of Cincinnati, Engineering and Applied Science: Electrical Engineering.
Sorting and purification of target cells from complex cellular samples is critical for downstream cell biology research, biomedical research and clinical diagnostics. In this work, a novel microfluidic platform is developed to achieve size-based sorting of cells with high performance and systematic versatility. The platform uses inertial effect of fluid and microscale vortices in designed microfluidic units to align and sort cells. A microfluidic unit was first developed to enable bimodal sorting of cells with high sorting resolution and tunability. Microparticle mixture with only 2 µm difference was continuously sorted with > 90% efficiency. Two sorting units were then integrated into specific arrangements to enable versatile cell sorting functions. A serial sequencing arrangement enabled new multimodal sorting function. Multi-sized or heterogeneous microparticle mixtures were sorted continuously into three subpopulations of different sizes with > 90% efficiency. Alternative arrangement of the two units enabled new double sorting and purification function. The larger target cells can be extracted from the small non-target cells two times sequentially yielding highly purified target cell product. Spiked human cancer stem-like cells (HuSLCs) were sorted from human blood samples with enriched concentration and significantly enhanced purity. The two distinct arrangements exhibit the systematic versatility of the vortex cell sorting platform for efficient sorting of complex cellular samples which will open new opportunities for size-based cellular sample preparation in cell biology, biomedical research and clinical diagnostics.
Ian Papautsky, Ph.D. (Committee Chair)
Chong Ahn, Ph.D. (Committee Member)
Chia Chi Ho, Ph.D. (Committee Member)
Susan Kasper, Ph.D. (Committee Member)
Andrew Steckl, Ph.D. (Committee Member)
100 p.
Biomedical Research
Microfluidics; Inertial microfluidics; Cell sorting; clinical diagnostics; Lab on a chip; Sample preparation

Recommended Citations

Citations

  • Wang, X. (2016). Inertial microfluidic vortex cell sorter [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1458643998

    APA Style (7th edition)

  • Wang, Xiao. Inertial microfluidic vortex cell sorter. 2016. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1458643998.

    MLA Style (8th edition)

  • Wang, Xiao. "Inertial microfluidic vortex cell sorter." Doctoral dissertation, University of Cincinnati, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1458643998

    Chicago Manual of Style (17th edition)

ucin1458643998
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© 2016, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.