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The Utilization of Fluorescent Products to Detect the Inhibition of Acetylcholinesterase by Carbamates and Organophosphate Pesticides: The Groundwork For a New Assay

Dussex, Dusten T
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1470743777

Abstract Details

2016, MS, University of Cincinnati, Medicine: Industrial Hygiene (Environmental Health).
Acetylcholinesterase (AChE) inhibitors such as organophosphate insecticides and carbamate insecticides have a severe effect on the human nervous system and other functions of the body. It is important to be able to quickly and accurately monitor water sources near agriculture for contamination by these pesticides. This thesis details the early stages of assay development for the detection of AChE inhibitors with a method that utilizes the formation of fluorescent products from the activity of the enzyme. AChE converts 2-naphthyl acetate to form 2-naphthol, a highly fluorescent product, which was detected via high performance liquid chromatography and fluorescence spectrometry (HPLC/FS). The formation of this product is limited as the enzyme activity is inhibited. This inhibition is quantified through the quantification of 2-naphthol formed. Though statistical significance was not analyzed, inhibition percentages of 5.5-70.9% were found for levels of aldicarb, a carbamate pesticide and potent inhibitor of AChE, from 0.0165 to 3.3 ppm. This assay is within an order of magnitude of target sensitivity based on EPA drinking standards, and has room for improvement for sensitivity as well as precision.
Mary Beth Genter, Ph.D. (Committee Chair)
Changchun Xie, Ph.D. (Committee Member)
30 p.
Environmental Health
Acetylcholinesterase Inhibition Detection; Organophosphates; Carbamates; Aldicarb; 2-Naphthol; HPLC and Fluorescence Spectrometry

Recommended Citations

Citations

  • Dussex, D. T. (2016). The Utilization of Fluorescent Products to Detect the Inhibition of Acetylcholinesterase by Carbamates and Organophosphate Pesticides: The Groundwork For a New Assay [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1470743777

    APA Style (7th edition)

  • Dussex, Dusten. The Utilization of Fluorescent Products to Detect the Inhibition of Acetylcholinesterase by Carbamates and Organophosphate Pesticides: The Groundwork For a New Assay. 2016. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1470743777.

    MLA Style (8th edition)

  • Dussex, Dusten. "The Utilization of Fluorescent Products to Detect the Inhibition of Acetylcholinesterase by Carbamates and Organophosphate Pesticides: The Groundwork For a New Assay." Master's thesis, University of Cincinnati, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1470743777

    Chicago Manual of Style (17th edition)

ucin1470743777
464
© 2016, some rights reserved.Creative Commons License The Utilization of Fluorescent Products to Detect the Inhibition of Acetylcholinesterase by Carbamates and Organophosphate Pesticides: The Groundwork For a New Assay by Dusten T Dussex is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Cincinnati and OhioLINK.