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Point-of-care Sensors for Determination of Manganese in Clinical Applications

Kang, Wenjing
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1471346294

Abstract Details

2016, PhD, University of Cincinnati, Engineering and Applied Science: Electrical Engineering.
Chronic exposure to elevated levels of manganese (Mn) can lead to a wide range of health issues. Both the World Health Organization (WHO) and the US Agency for Toxic Substances and Disease Registry (ATSDR) have established safely limits for Mn in water and blood. To protect public health and the environment, it is critically important to monitor concentrations of Mn, so that clinicians and environmental scientists can take immediate action when necessary. The conventional approaches to monitoring Mn, such as ICP-MS or AAS, are extremely accurate, but are costly and are not suitable for fast response. A point-of-care system featuring electrochemical sensing can meet the requirements of a simple, cost-efficient, but highly sensitive analytical tool. This dissertation describes development of a microscale electrochemical sensor for determination of Mn using cathodic stripping voltammetry. The explored sensors include a copper (Cu)-based sensor with palladium (Pd) film working electrode, a platinum (Pt) sensor, and an indium tin oxide (ITO) sensor. With proper characterization of electrodes and optimization of parameters, a sufficiently low limit of detection (LOD) can be achieved. For example, the Cu-Pd sensor on glass substrate demonstrated LOD of 333.6 nM (18.3 ppb) in pH 9.0, 0.1 M borate buffer. For the Pt sensor, the LOD improved to 16.3 nM (0.9 ppb) in pH 5.5, 0.2 M acetate buffer, with similar performance for the ITO sensor. Using the Pt sensor, trace Mn concentrations were determined in natural water samples (pond water, river water, and well water) and achieved >97% precision and ~90% accuracy when we compared with ICP-MS. Using the ITO sensor, Mn was determined in digested whole blood samples. Ultimately, these results suggest that electrochemical sensors can enable sensitive and accurate on-site determination of Mn in various sample matrices. With additional development, these sensors can be integrated into a user-friendly portable system, which could potentially become a viable alternative to the conventional lab-based analysis for clinicians and environmental scientists.
Ian Papautsky, Ph.D. (Committee Chair)
Adam F. Bange, Ph.D. (Committee Member)
Erin Nicole Haynes, Dr.P.H. (Committee Member)
William Heineman, Ph.D. (Committee Member)
Andrew Steckl, Ph.D. (Committee Member)
Philip Wilsey, Ph.D. (Committee Member)
92 p.
Analytical Chemistry
Electrochemical sensors; Cathodic Stripping voltammetry; Manganese determination; Point-of-care; Environmental applications; Clinical applications

Recommended Citations

Citations

  • Kang, W. (2016). Point-of-care Sensors for Determination of Manganese in Clinical Applications [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1471346294

    APA Style (7th edition)

  • Kang, Wenjing. Point-of-care Sensors for Determination of Manganese in Clinical Applications. 2016. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1471346294.

    MLA Style (8th edition)

  • Kang, Wenjing. "Point-of-care Sensors for Determination of Manganese in Clinical Applications." Doctoral dissertation, University of Cincinnati, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1471346294

    Chicago Manual of Style (17th edition)

ucin1471346294
266
© 2016, some rights reserved.Creative Commons License Point-of-care Sensors for Determination of Manganese in Clinical Applications by Wenjing Kang is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Cincinnati and OhioLINK.