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Principal Component Analysis Approach for Determination of Stroke Protein Biomarkers and Modified Atmospheric Pressure Chemical Ionization Source Development for Volatile Analyses

Nahan, Keaton
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1491817651627161

Abstract Details

2017, PhD, University of Cincinnati, Arts and Sciences: Chemistry.
Proteins play a role in neurological health and disorders. About 25% of proteins contain metals and these proteins are known as metalloproteins. Both blood plasma proteins and metalloproteins play an active role in stroke. These proteins may be quantified with approaches as simple as multiplexed assay to that of quantitative protein mass spectrometry (proteomics). Identification of present bound metals is more elusive. Inductively coupled plasma mass spectrometry (ICP-MS) is capable of both quantitation as well as speciation of metalloproteins when coupled with chromatography. Determination of metalloproteins, for use as a stroke determination tool, was performed by bottom up proteomics. Multivariate analysis was used to simplify the determination of important stroke biomarkers that were identified or quantified by quantitative multiplexed assays and tandem mass spectrometry, respectively. While ICP-MS was used as a means for determination of metal containing species, atmospheric pressure chemical ionization mass spectrometry (APCI-MS) is used for quantitation of volatile species. Typical means of APCI-MS are coupled with chromatography for separations prior to quantitation or directly performed by flow injection. Alternatively, gas chromatography mass spectrometry (GC-MS) also is capable of separating volatile molecules at low levels by using solid phase microextraction (SPME) fibers. In order to improve APCI-MS, it was proposed to modify an APCI source to incorporate a conductive SPME source that was capable of low solvent analysis for detection of volatile species. While commercial SPME fibers are produced from a variety of polymers, this modified APCI source was produced from multiwalled carbon nanotubes (MWCNT’s) to ensure flexibility, improved surface area, and conductivity.
Julio Landero Figueroa, Ph.D. (Committee Chair)
Peng Zhang, Ph.D. (Committee Chair)
Thomas Ridgway, Ph.D. (Committee Member)
George Stan, Ph.D. (Committee Member)
143 p.
Chemistry
Stroke; Metallomics; Multivariate Statistics; Mass Spectrometry; Carbon Nanotubes; SPME

Recommended Citations

Citations

  • Nahan, K. (2017). Principal Component Analysis Approach for Determination of Stroke Protein Biomarkers and Modified Atmospheric Pressure Chemical Ionization Source Development for Volatile Analyses [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1491817651627161

    APA Style (7th edition)

  • Nahan, Keaton. Principal Component Analysis Approach for Determination of Stroke Protein Biomarkers and Modified Atmospheric Pressure Chemical Ionization Source Development for Volatile Analyses. 2017. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1491817651627161.

    MLA Style (8th edition)

  • Nahan, Keaton. "Principal Component Analysis Approach for Determination of Stroke Protein Biomarkers and Modified Atmospheric Pressure Chemical Ionization Source Development for Volatile Analyses." Doctoral dissertation, University of Cincinnati, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1491817651627161

    Chicago Manual of Style (17th edition)

ucin1491817651627161
421
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This open access ETD is published by University of Cincinnati and OhioLINK.