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Optimization of Methods for Generating Customized Gene-Edited Human Pluripotent Stem Cells

Campbell, Ian
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1504802720510926

Abstract Details

2017, MS, University of Cincinnati, Medicine: Molecular and Developmental Biology.
Human pluripotent stem cells (hPSCs) have the capacity for self-renewal and the ability to differentiate into any cell type providing an unlimited source of primary human cells capable of differentiation. Consequently, hPSCs have diverse applications ranging from novel lines for research in human development to the production of biological products for regenerative medicine. Improvements in gene editing technologies have expanded the potential applications of hPSCs, granting the ability to produce novel cell lines such as isogenic pairs of edited and unedited hPSC lines for gene function studies, cell lines with specific disease mutations, or reporter lines for functional studies and lineage tracing. Current methods for gene editing hPSCs are insufficiently robust for routine genome editing of diverse hPSCs. Costs and the time needed associated with optimization of standard gene editing practices may prove restrictive in many laboratories. The study reported in this thesis describe the optimization of standard CRISPR/cas9 genome engineering methods for of iPSCs, resulting in the development of a robust and broadly applicable procedure for routinely gene editing multiple iPSC lines.
Rashmi Hegde, Ph.D. (Committee Chair)
Samantha Brugmann, Ph.D. (Committee Member)
Chris Mayhew, Ph.D. (Committee Member)
Kaushik Roychoudhury, Ph.D. (Committee Member)
8 p.
Biomedical Research
iPSC; CRISPR; gene editing; Pluripotent stem cell; cas9

Recommended Citations

Citations

  • Campbell, I. (2017). Optimization of Methods for Generating Customized Gene-Edited Human Pluripotent Stem Cells [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1504802720510926

    APA Style (7th edition)

  • Campbell, Ian. Optimization of Methods for Generating Customized Gene-Edited Human Pluripotent Stem Cells. 2017. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1504802720510926.

    MLA Style (8th edition)

  • Campbell, Ian. "Optimization of Methods for Generating Customized Gene-Edited Human Pluripotent Stem Cells." Master's thesis, University of Cincinnati, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1504802720510926

    Chicago Manual of Style (17th edition)

ucin1504802720510926
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© 2017, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.