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Molecular Characterization and Endocrine Regulation of Development in Tadpoles of Xenopus Tropicalis

Schneider, Katelin A
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1511858507180701

Abstract Details

2017, MS, University of Cincinnati, Arts and Sciences: Biological Sciences.
Hormones, specifically stress hormones and thyroid hormone, are required for proper growth and development during the perinatal period. Deviations in normal hormone levels, arising from either environment changes or congenital diseases, can cause deleterious alterations in organ maturation and timing of birth. These deviations can also lead to irreversible later life consequences, such a cardiovascular disease and weakened immune function. To study how hormones effect development, we use tadpoles as a model due to their exquisite hormone sensitivity and dependence on thyroid hormone and corticosteroids for development. Chapter 1 entails characterizing a stress hormone response gene. We confirm that a previously found transcribed locus Str. 34945 is induced by the stress hormone corticosterone (CORT), and is known to be the only CORT only response gene in Xenopus tropicalis tadpoles. In this chapter, we examine time course induction, tissue distribution, and the expression of this gene over metamorphosis. We also confirm the genomic annotation of this transcript, showing Str. 34945 is a part of the gene ush1g. Use of this gene will greatly facilitate future mechanistic studies on how stress hormone response genes effect development. Chapter 2 focuses on how endocrine disrupting compounds (EDCs) can affect normal development. We looked at how Bisphenol S (BPS), which is a common substitute for Bisphenol A (BPA) in “BPA free products” effects the thyroid hormone pathway in Xenopus tropicalis tadpoles. We investigated if exposure to BPS can lead to expression alterations of thyroid hormone response genes. We also looked at how long term exposure to BPS effects tadpole growth during metamorphosis. Broadly, this work provides a tool to help determine the effects of stress on development. This work also paves way for future studies to determine how EDCs can lead to deviations in development
Daniel Buchholz, Ph.D. (Committee Chair)
Joshua Benoit, Ph.D. (Committee Member)
Edwin Griff, Ph.D. (Committee Member)
55 p.
Biology
Metamorphosis; Gene expression; Development; Endocrine disruptors

Recommended Citations

Citations

  • Schneider, K. A. (2017). Molecular Characterization and Endocrine Regulation of Development in Tadpoles of Xenopus Tropicalis [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1511858507180701

    APA Style (7th edition)

  • Schneider, Katelin. Molecular Characterization and Endocrine Regulation of Development in Tadpoles of Xenopus Tropicalis. 2017. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1511858507180701.

    MLA Style (8th edition)

  • Schneider, Katelin. "Molecular Characterization and Endocrine Regulation of Development in Tadpoles of Xenopus Tropicalis." Master's thesis, University of Cincinnati, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1511858507180701

    Chicago Manual of Style (17th edition)

ucin1511858507180701
141
© 2017, some rights reserved.Creative Commons License Molecular Characterization and Endocrine Regulation of Development in Tadpoles of Xenopus Tropicalis by Katelin A Schneider is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Cincinnati and OhioLINK.