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Role of Glucocorticoid Signaling in Regulation of Amphibian Metamorphosis

Shewade, Leena H.
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1535466761073155

Abstract Details

2018, PhD, University of Cincinnati, Arts and Sciences: Biological Sciences.
Amphibian metamorphosis has been extensively used as a model system to study the developmental actions of thyroid hormone (TH) and corticosterone (CORT). While TH is necessary and sufficient to induce metamorphosis, CORT can modulate the developmental progression depending on timing and circulating levels of TH in the system. Both hormones have several pleiotropic and interactive effects that have been difficult to isolate due to lack of technological advances in the past. In my thesis, I examined the regulation of the kruppel like factor 9 (KLF9) by corticosteroids in tadpole tissues across development. I also establish a novel role of CORT in metamorphic completion and investigate its function in regulation of tissue sensitivity and responsivity to TH. In chapter 2, I investigated the ability of the corticosteroid hormones – Aldosterone (ALDO) and CORT to upregulate klf9. Then I quantified the developmental profiles of the corticosteroid nuclear receptors – type I or mineralocorticoid receptor (MR) and type-II or glucocorticoid receptor (GR) and determined their role in klf9 regulation by corticosteroids. In chapter 3, I used ACTH knockout X. tropicalis and revealed a novel and critical role for CORT in metamorphic survival. This lethality stems from a potential respiratory distress after gill resorption and can be rescued by CORT treatment. The ACTH knockout was extensively characterized at the metabolic, morphological and gene expression level. In Chapter 4, I examined the effect of curtailed CORT levels in ACTH knockout X. tropicalis on the rate of growth and development. The reduced growth and delayed developmental were associated with impairment in peripheral TH signaling as determined from reduced target gene expressions, tissue responsivity to exogenous TH and abnormal levels of deiodinases.
Daniel Buchholz, Ph.D. (Committee Chair)
Edwin Griff, Ph.D. (Committee Member)
Dennis Grogan, Ph.D. (Committee Member)
Joshua Gross, Ph.D. (Committee Member)
Aaron Zorn, Ph.D. (Committee Member)
126 p.
Developmental Biology
amphibian metamorphosis; hormones in development; glucocorticoids in metamorphosis

Recommended Citations

Citations

  • Shewade, L. H. (2018). Role of Glucocorticoid Signaling in Regulation of Amphibian Metamorphosis [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1535466761073155

    APA Style (7th edition)

  • Shewade, Leena. Role of Glucocorticoid Signaling in Regulation of Amphibian Metamorphosis. 2018. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1535466761073155.

    MLA Style (8th edition)

  • Shewade, Leena. "Role of Glucocorticoid Signaling in Regulation of Amphibian Metamorphosis." Doctoral dissertation, University of Cincinnati, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1535466761073155

    Chicago Manual of Style (17th edition)

ucin1535466761073155
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This open access ETD is published by University of Cincinnati and OhioLINK.