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Biocompatible noble metal nanoparticle substrates for bioanalytical and biophysical analysis of protein and lipids

Bruzas, Ian R
http://orcid.org/0000-0002-9098-1572
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1553250462519941

Abstract Details

2019, PhD, University of Cincinnati, Arts and Sciences: Chemistry.
Noble metal nanoparticles are excellent candidates for sensing and biophysical characterization of lipid membranes and proteins, as they offer high sensitivity and are label-free. Noble metal nanoparticles facilitate sensing predominantly through two-mechanisms: the localized surface plasmon resonance (LSPR) and surface-enhanced Raman scattering (SERS). Both methods are robust well-developed sensing methods that require the analyte to be positioned at or near the metal nanoparticle surface. The interaction of biomolecules such as protein and lipid bilayers with metal surfaces, however, is often perturbative and can alter the structure and function of biomolecules. Thus, for biomolecular sensing with metal nanoparticles it is essential to develop substrates that impart biocompatibility while maintaining sensitivity. This work reports on the development of substrates composed of gold or silver nanoparticles that are passivated with ultrathin silica films to enable the biocompatible measurement of supported lipid bilayers and towards the biocompatible measurement of protein. A silica sol-gel method was developed to prepare thin films over nanodisks for the quantitation of cholera toxin binding to gm1 containing lipid bilayers. A modified sol-gel procedure was used to coat silver film over nanosphere substrates with silica to enable SERS measurement of supported lipid bilayer formation and lipid exchange. In addition to silica coating strategies a liposome-based nanoparticle-on-mirror substrate was developed to measure dye molecules contained within a liposome using SERS, which may be useful for the biocompatible measurement of single protein molecules by SERS. Finally, liposomes coated with metal nanoparticles and metal nanoshells were developed for their potential use as dynamic SERS biosensors.
Laura Sagle, Ph.D. (Committee Chair)
Michael Baldwin, Ph.D. (Committee Member)
Patrick Limbach, Ph.D. (Committee Member)
138 p.
Chemistry
noble metal nanoparticles; biosensing; plasmonics; surface enhanced Raman spectroscopy; supported lipid bilayer; biophysics

Recommended Citations

Citations

  • Bruzas, I. R. (2019). Biocompatible noble metal nanoparticle substrates for bioanalytical and biophysical analysis of protein and lipids [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1553250462519941

    APA Style (7th edition)

  • Bruzas, Ian. Biocompatible noble metal nanoparticle substrates for bioanalytical and biophysical analysis of protein and lipids. 2019. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1553250462519941.

    MLA Style (8th edition)

  • Bruzas, Ian. "Biocompatible noble metal nanoparticle substrates for bioanalytical and biophysical analysis of protein and lipids." Doctoral dissertation, University of Cincinnati, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1553250462519941

    Chicago Manual of Style (17th edition)

ucin1553250462519941
367
© 2019, some rights reserved.Creative Commons License Biocompatible noble metal nanoparticle substrates for bioanalytical and biophysical analysis of protein and lipids by Ian R Bruzas is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Cincinnati and OhioLINK.