Skip to Main Content
Frequently Asked Questions
Submit an ETD
Global Search Box
Need Help?
Keyword Search
Participating Institutions
Advanced Search
School Logo
Files
File List
40496.pdf (1.14 MB)
ETD Abstract Container
Abstract Header
Limited Capacity of Fetal Neutrophils to Form Extracellular Traps
Author Info
Thompson, Ravyn
ORCID® Identifier
http://orcid.org/0000-0003-1719-3876
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1637157011170823
Abstract Details
Year and Degree
2021, MS, University of Cincinnati, Medicine: Immunology.
Abstract
NETosis is a regulated form of cell death that neutrophils initiate in order to trap and kill bacteria. During this process neutrophil extracellular traps (NETs) are released. The traps consist of extracellularized DNA coated with antimicrobial proteins. Additionally, the release and rupture of granulocytes helps kill foreign bodies. Little is known about NET formation in the context of fetal infection and inflammation. During in utero infection, fetal lungs and other mucosal tissues come in contact with bacteria and bacterial products present in the amniotic fluid. To study the functionality of fetal neutrophils, two Rhesus macaque models of chorioamnionitis were used. Pregnant animals were injected intra-amniotically with lipopolysaccharide (LPS) or Escherichia coli (E. coli), and fetal lung tissues were harvested after 16 or 48 hours respectively. NETs were identified by immunohistochemistry detection of extracellular citrullinated histone in neutrophils, identified as CD68+HLA-DRneg cells. Compared to controls, the IA LPS fetal lungs did have significantly greater mean NET area. To compare the functionality of fetal neutrophils to adult neutrophils, fetal lung samples were matched with their corresponding chorioamnion. In LPS animals, there was a trend towards higher NET formation in the chorioamnion, suggesting that fetal neutrophils may be capable of NET formation but not as much as adult neutrophils. We also compared the LPS treatment to the E. coli treatment since NETs are classically thought to trap and kill live bacteria. We found no difference between the two treatments demonstrating that fetal neutrophils respond to bacterial products.
Committee
Claire Chougnet, Ph.D. (Committee Chair)
Ian Lewkowich (Committee Member)
Jonathan Katz, Ph.D. (Committee Member)
Pages
32 p.
Subject Headings
Immunology
Keywords
NETs
;
neutrophil extracellular traps
;
Rhesus macaque
;
Rhesus
;
neutrophils
;
LPS
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
Thompson, R. (2021).
Limited Capacity of Fetal Neutrophils to Form Extracellular Traps
[Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1637157011170823
APA Style (7th edition)
Thompson, Ravyn.
Limited Capacity of Fetal Neutrophils to Form Extracellular Traps.
2021. University of Cincinnati, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1637157011170823.
MLA Style (8th edition)
Thompson, Ravyn. "Limited Capacity of Fetal Neutrophils to Form Extracellular Traps." Master's thesis, University of Cincinnati, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1637157011170823
Chicago Manual of Style (17th edition)
Abstract Footer
Document number:
ucin1637157011170823
Download Count:
37
Copyright Info
© 2021, some rights reserved.
Limited Capacity of Fetal Neutrophils to Form Extracellular Traps by Ravyn Thompson is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Cincinnati and OhioLINK.