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VGLUT and GAD65 Expression in Physiologically Characterized Ia Afferents

Ukpabi, Ivonne Nkoli
http://rave.ohiolink.edu/etdc/view?acc_num=wright1199638189

Abstract Details

2007, Master of Science (MS), Wright State University, Physiology and Biophysics.
Peripheral nerve injury is known to induce several changes in the physiology and morphology of the injured afferent. These changes include hyperexcitability, decreased dorsal root potentials (DRP), loss of synaptic vesicles as well as loss of the vesicular glutamate transporter, VGLUT1. While many of the changes caused by peripheral injury revert with regeneration, others appear to be permanent. The loss of the stretch reflex for example is a puzzling outcome of recovery after peripheral nerve transection and regeneration, especially given that the electrical counterpart of the stretch reflex (the H-reflex) recovers after regeneration. We hypothesized that stretch induced transmission is suppressed at the primary afferent – motor neuron synapse and sought to determine whether this was due to a decrease in excitatory transmission or an increase in presynaptic inhibition. To this end, we measured the level of expression of VGLUT1 and 2 as well as the GABA synthesizing enzyme GAD65 in the central terminals of physiologically characterized primary afferents from healthy Wistar rats for comparison with regenerated animals. An inverse relationship appears to exist between VGLUT1 and GAD65 expression. Expression of the two proteins also differ in laminae V, VII and IX, with the highest VGLUT1 immunoreactivity in lamina V and the highest number of GAD65-intense presynaptic boutons in lamina IX. The possible effects of regeneration on this pattern are discussed.
Timothy Cope (Advisor)
78 p.
VGLUT1; VGLUT2; GAD65; Ia afferent

Recommended Citations

Citations

  • Ukpabi, I. N. (2007). VGLUT and GAD65 Expression in Physiologically Characterized Ia Afferents [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1199638189

    APA Style (7th edition)

  • Ukpabi, Ivonne. VGLUT and GAD65 Expression in Physiologically Characterized Ia Afferents. 2007. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1199638189.

    MLA Style (8th edition)

  • Ukpabi, Ivonne. "VGLUT and GAD65 Expression in Physiologically Characterized Ia Afferents." Master's thesis, Wright State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=wright1199638189

    Chicago Manual of Style (17th edition)

wright1199638189
828
© 2007, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.