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wright1205197070.pdf (2.29 MB)
ETD Abstract Container
Abstract Header
QTL analysis of ray pattern in Caenorhabditis elegans recombinant inbred lines
Author Info
Guess, Adam Joseph
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=wright1205197070
Abstract Details
Year and Degree
2008, Master of Science (MS), Wright State University, Biological Sciences.
Abstract
Ray pattern is the most divergent morphological character among rhabditid nematodes. The ancestral ray pattern of Elegans group nematodes is commonly referred to as a 2(1)3+3 pattern, with rays 1 and 2 located anterior of the cloaca, ray 3 positioned at the cloaca, and rays 4-6 and 7-9 grouped in two clusters posterior of the cloaca. C. elegans males primarily exhibit the 2(1)3+3 pattern; however some isolates display variant patterns at a low frequency. The Hawaiian strain CB4856 exhibits a 3/3+3 pattern at a frequency of 0.09. In this pattern, ray 3 is located anterior of the cloaca in a cluster with rays 1 and 2. The designated wild-type strain, N2, is fixed for the 2(1)3+3 pattern. To examine the genetic basis of this phenotypic variation, recombinant inbred lines (RIL) derived from a ten-generation intercross between strains N2 and CB4856 were scored for ray pattern. Transgressive segregation was observed with the 3/3+3 ray pattern being expressed at much higher frequencies in many RIL than in either parental strain. QTL analyses of ray pattern data revealed a single highly significant QTL on the left side of chromosome V (LOD score = 14.273; p = 1.02e-10) as well as a marginally significant QTL on the right side of chromosome I (LOD score = 3.017; p = 0.109). To confirm the major QTL, CB4856 alleles from the left end of chromosome V were introgressed into an otherwise N2 background. From these data, a 500 Kb region of chromosome V was defined that has a major impact on the frequency of the 3/3+3 ray pattern.
Committee
Scott Baird, PhD (Advisor)
Barbara Hull, PhD (Committee Member)
Dan Krane, PhD (Committee Member)
Pages
64 p.
Subject Headings
Biology
;
Genetics
Keywords
C. elegans
;
QTL mapping
;
ray pattern
;
N2
;
CB4856
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Citations
Guess, A. J. (2008).
QTL analysis of ray pattern in Caenorhabditis elegans recombinant inbred lines
[Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1205197070
APA Style (7th edition)
Guess, Adam.
QTL analysis of ray pattern in Caenorhabditis elegans recombinant inbred lines.
2008. Wright State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=wright1205197070.
MLA Style (8th edition)
Guess, Adam. "QTL analysis of ray pattern in Caenorhabditis elegans recombinant inbred lines." Master's thesis, Wright State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=wright1205197070
Chicago Manual of Style (17th edition)
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Document number:
wright1205197070
Download Count:
935
Copyright Info
© 2008, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.