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Immunomodulation of Human Skin Cells by Extracts of the Scabies Mite, Sarcoptes scabiei

Mullins, Jeremi Stevan
http://rave.ohiolink.edu/etdc/view?acc_num=wright1214591687

Abstract Details

2008, Master of Science (MS), Wright State University, Biological Sciences.
Sarcoptes scabiei infests its host through burrowing into the stratum corneum layer of skin. Keratinocytes and fibroblasts responding to the burrowing mite(s) and/or mite products produce inflammatory and immune activating cytokines and chemokines. The purpose of this study was to determine if an extract of S. scabiei can modulate cytokine and chemokine expression from keratinocytes and fibroblasts in vitro. Normal human keratinocytes and fibroblasts were exposed to an extract of S. scabiei var. canis with or without various pro-inflammatory stimuli. Cytokine and chemokine expression were measured by enzyme-linked immunosorbent assay (ELISA). Scabies extract increased expression of interleukin-6 (IL-6), growth-related oncogene-α (GRO-α), transforming growth factor-α (TGF-α), and cutaneous T-cell attracting chemokine (CTACK) by keratinocytes. In contrast, scabies extract decreased expression of interleukin-8 (IL-8) by keratinocytes. In comparison, scabies extract increased expression of IL-6 and granulocyte-colony stimulating factor (G-CSF) by fibroblasts. In contrast, scabies extract decreased expression of granulocyte/macrophage-colony stimulating factor (GM-CSF) and IL-8 by fibroblasts. These findings demonstrate that S. scabiei can modulate cytokine and chemokine expression by human keratinocytes and fibroblasts.
Larry G. Arlian, PhD (Advisor)
James McDougal, PhD (Committee Member)
Mill Miller, PhD (Committee Member)
142 p.
Immunology
Cytokines; Scabies; Keratinocytes; Fibroblasts

Recommended Citations

Citations

  • Mullins, J. S. (2008). Immunomodulation of Human Skin Cells by Extracts of the Scabies Mite, Sarcoptes scabiei [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1214591687

    APA Style (7th edition)

  • Mullins, Jeremi. Immunomodulation of Human Skin Cells by Extracts of the Scabies Mite, Sarcoptes scabiei. 2008. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1214591687.

    MLA Style (8th edition)

  • Mullins, Jeremi. "Immunomodulation of Human Skin Cells by Extracts of the Scabies Mite, Sarcoptes scabiei." Master's thesis, Wright State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=wright1214591687

    Chicago Manual of Style (17th edition)

wright1214591687
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© 2008, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.