Skip to Main Content
 

Global Search Box

 
 
 
 

ETD Abstract Container

Abstract Header

NMR analyses show TCDD elicits differences in hepatic metabolism in female C57BL/6 mice and Sprague-Dawley rats

Makley, Meghan Katherine

Abstract Details

2008, Master of Science (MS), Wright State University, Biochemistry and Molecular Biology.

TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) elicits tissue-, sex-, and species-specific effects. This study compares the hepatic response to an oral dose of TCDD in immature ovariectomized (i.o.) C57BL/6 mice (30 µg/kg) and i.o. Sprague-Dawley rats (10 µg/kg), at 72, 120, and 168 h post-dose. Hepatic lipid extracts were analyzed by 13C and 31P NMR, and aqueous extracts by 1H and 31P NMR.

Consistent with increased lipid content in mice (p≤0.05), TCDD induced increases in hepatic triacylglycerides (TAG), cholesterol, and fatty acids. Principal component analysis of 13C spectra show treatment groups separate in mice, but not rats. Mice showed decreases in the lactate/pyruvate ratio and dihydroxyacetone phosphate, consistent with decreased cytosolic NADH/NAD+ ratio and upregulated TAG synthesis. TCDD-treated rats exhibited decreased levels of sphingomyelin, and three-fold increase in phosphocholine, suggesting TCDD activates sphingomyelinase. Both species showed similar decreases in cardiolipin, indicating oxidative stress. These observations are compared with hepatic histopathology and gene expression findings.

Nicholas Reo, PhD (Advisor)
Nicholas Reo, PhD (Committee Chair)
Darrell Fleischman, PhD (Committee Member)
Lawrence Prochaska, PhD (Committee Member)
100 p.

Recommended Citations

Citations

  • Makley, M. K. (2008). NMR analyses show TCDD elicits differences in hepatic metabolism in female C57BL/6 mice and Sprague-Dawley rats [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1230048333

    APA Style (7th edition)

  • Makley, Meghan. NMR analyses show TCDD elicits differences in hepatic metabolism in female C57BL/6 mice and Sprague-Dawley rats. 2008. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1230048333.

    MLA Style (8th edition)

  • Makley, Meghan. "NMR analyses show TCDD elicits differences in hepatic metabolism in female C57BL/6 mice and Sprague-Dawley rats." Master's thesis, Wright State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=wright1230048333

    Chicago Manual of Style (17th edition)