Skip to Main Content
 

Global Search Box

 
 
 
 

ETD Abstract Container

Abstract Header

DOSE-DEPENDENT EFFECTS OF OXYGEN ON METABOLISM IN RAT CORTICO-HIPPOCAMPAL BRAIN TISSUE SLICES

HOLLYFIELD, JENNIFER LYNNE

Abstract Details

2009, Master of Science (MS), Wright State University, Biochemistry and Molecular Biology.
Studies have shown that 95% oxygen increases neuronal excitability and ROS production. We wanted to investigate the dose-dependent effects of oxygen on brain slice metabolism. We exposed rat brain cortico-hippocampal tissue slices to 0.40, 0.95, and 4.50 ATA O2 for 60 minutes, made dual-phase tissue extracts, and used multi-nuclear NMR experiments to elucidate the slice metabolism. We found that low doses of oxygen may shift metabolism toward anaerobic glycolysis. Elevated lactate suggests this shift, along with elevated ratios of NAD+/NADH which may drive the reactions toward the production of lactate. The results also suggest that high doses of oxygen may cause aerobic glycolysis and oxidative phosphorylation to accelerate. Lower lactate amounts along with a low NAD+/NADH ratio may be driving these reactions towards pyruvate and the TCA cycle and suggest a highly oxidized environment. The 0.40 ATA O2 results suggest a hypoxic environment while the 0.95 and 4.50 ATA O2 results suggest hyperoxic environments that lead to changes in metabolism on both ends of the oxygen spectrum.
Nicholas Reo, PhD (Advisor)
Lawrence Prochaska, PhD (Committee Member)
John Paietta, PhD (Committee Member)
67 p.

Recommended Citations

Citations

  • HOLLYFIELD, J. L. (2009). DOSE-DEPENDENT EFFECTS OF OXYGEN ON METABOLISM IN RAT CORTICO-HIPPOCAMPAL BRAIN TISSUE SLICES [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1242417163

    APA Style (7th edition)

  • HOLLYFIELD, JENNIFER. DOSE-DEPENDENT EFFECTS OF OXYGEN ON METABOLISM IN RAT CORTICO-HIPPOCAMPAL BRAIN TISSUE SLICES. 2009. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1242417163.

    MLA Style (8th edition)

  • HOLLYFIELD, JENNIFER. "DOSE-DEPENDENT EFFECTS OF OXYGEN ON METABOLISM IN RAT CORTICO-HIPPOCAMPAL BRAIN TISSUE SLICES." Master's thesis, Wright State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=wright1242417163

    Chicago Manual of Style (17th edition)