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Identification of Potential Protein Biomarkers of Low Level Kidney Degradation

Woolard, Christopher Lee
http://rave.ohiolink.edu/etdc/view?acc_num=wright1247498817

Abstract Details

2009, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
The purpose of this research was the identification of potential dose-dependent and time-dependent serum protein biomarkers of low level kidney degradation in a rat model. Potential biomarkers were evaluated based on differential protein expression between control and dosed samples in rat serum. Proteins of interest demonstrated upregulation at a minimum 1.5 fold increase in protein concentration control versus dosed sample. In order to identify common biomarkers of kidney decrement, three nephrotoxins were chosen to target specific locations of the kidney: 1) D-Serine, which causes necrosis of the proximal straight tubules, 2) Puromycin, an antibiotic that degrades the Glomerular Basement Membrane (GBM), and 3) Bromoethyl Amine (BEA), which affects the proximal tubules as well. Rats were dosed with individual neprotoxins and serum collected at Pre-dose, 24 hrs Post dose, and at terminal sacrifice for Puromycin andBEA, and at 12 hrs and 24 hrs post-dose from d-serine. The collected serum sample was enriched to remove abundant proteins, separated using 2D Difference in Gel Electrophoresis (DIGE), and screened for potential up-regulated biomarkers based on difference in fluorescent intensity using computer software. Finally, the proteins were trypsin digested and the resultant peptides identified by MALDI-TOF/TOF mass spectroscopy. The study results indicated hornerin as a potential biomarker though no dose- or time-dependent toxicity could be determined.
Camilla Mauzy, PhD (Advisor)
David Cool, PhD (Committee Member)
Khalid Elased, PhD (Committee Member)
158 p.
Pharmacology; Toxicology
Biomarker; d-serine, puromycin; bromoethylamine; rat serum; immunodepletion; 2D-difference in gel electrophoresis

Recommended Citations

Citations

  • Woolard, C. L. (2009). Identification of Potential Protein Biomarkers of Low Level Kidney Degradation [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1247498817

    APA Style (7th edition)

  • Woolard, Christopher. Identification of Potential Protein Biomarkers of Low Level Kidney Degradation. 2009. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1247498817.

    MLA Style (8th edition)

  • Woolard, Christopher. "Identification of Potential Protein Biomarkers of Low Level Kidney Degradation." Master's thesis, Wright State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=wright1247498817

    Chicago Manual of Style (17th edition)

wright1247498817
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© 2009, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.