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INFLUENCE OF DIET ON POPULATION GROWTH AND ALLERGEN PRODUCTION IN CULTURED HOUSE DUST MITES - DERMATOPHAGOIDES FARINAE AND DERMATOPHAGOIDES PTERONYSSINUS

Poola, Swetha Avula
http://rave.ohiolink.edu/etdc/view?acc_num=wright1247665992

Abstract Details

2009, Master of Science (MS), Wright State University, Biological Sciences.
Dermatophagoides farinae and Dermatophagoides pteronyssinus culture extracts are used for skin testing, immunotherapy and research studies. There is a need to develop an effective culture method so that standardized extracts and allergens can be produced. The purpose of this study was to examine if different diets effect mite population growth and the amount of allergen produced in cultures. Dermatophagoides farinae and Dermatophagoides pteronyssinus were cultured on diets A and B. The density of mites, life stage composition and allergen concentration was determined at two week intervals. Enzyme-linked immune sorbent assays (ELISA) were used to assess group 1 and group 2 allergen concentrations. This study showed that diet is an important factor for culturing of mites. Diet B was found to be better suited to culture Dermatophagoides farinae whereas diet A was better for culturing of Dermatophagoides pteronyssinus. Diet A cultures of Dermatophagoides pteronyssinus produced greater concentrations of group 2 allergen than diet B.
Larry Arlian, PhD (Advisor)
Barbara Hull, PhD (Committee Member)
Scott Baird, PhD (Committee Member)
116 p.
Biology
MITES; ALLERGEN; DIET; DERMATOPHAGOIDES; FARINAE; PTERONYSSINUS; grown on diet

Recommended Citations

Citations

  • Poola, S. A. (2009). INFLUENCE OF DIET ON POPULATION GROWTH AND ALLERGEN PRODUCTION IN CULTURED HOUSE DUST MITES - DERMATOPHAGOIDES FARINAE AND DERMATOPHAGOIDES PTERONYSSINUS [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1247665992

    APA Style (7th edition)

  • Poola, Swetha. INFLUENCE OF DIET ON POPULATION GROWTH AND ALLERGEN PRODUCTION IN CULTURED HOUSE DUST MITES - DERMATOPHAGOIDES FARINAE AND DERMATOPHAGOIDES PTERONYSSINUS. 2009. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1247665992.

    MLA Style (8th edition)

  • Poola, Swetha. "INFLUENCE OF DIET ON POPULATION GROWTH AND ALLERGEN PRODUCTION IN CULTURED HOUSE DUST MITES - DERMATOPHAGOIDES FARINAE AND DERMATOPHAGOIDES PTERONYSSINUS." Master's thesis, Wright State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=wright1247665992

    Chicago Manual of Style (17th edition)

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© 2009, some rights reserved.Creative Commons License INFLUENCE OF DIET ON POPULATION GROWTH AND ALLERGEN PRODUCTION IN CULTURED HOUSE DUST MITES - DERMATOPHAGOIDES FARINAE AND DERMATOPHAGOIDES PTERONYSSINUS by Swetha Avula Poola is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.